Toxicity and genetic analysis of bees Scaptotrigona bipunctata after contamination with insecticide accephate
DOI:
https://doi.org/10.36560/13820201157Palavras-chave:
organophosphate, esterases, midgutResumo
The meliponines can be found in tropical and subtropical regions and is observed in the majority of Latin America. Scaptotrigona bipunctata are stingless bees that build colonies that possess between 2,000 and 50,000 individuals. This study aimed to estimate the toxicity of the insecticide acephate after oral and contact contamination in S. bipunctata. Results revealed differences between the two types of contamination and indicated that S. bipunctata is tolerant to acephate since the mortality rate was low. The findings indicated that there were expression changes of isoenzyme esterases after contamination with the insecticide, which was able to partially inhibit almost all of the enzymes identified in this study. In addition, morphological changes were identified in the midgut of the bees in the first 24 hours of contamination. However, after 48 hours it was observed the regeneration of the peritrophic membrane and other structures, contributing to the survival of the insects. It is essential to propose measures to minimize the impact of agrochemicals on pollinators and this study provides support for investigations on pesticide toxicity in stingless bees.
Referências
ABDALLA, F.C., JONES, G.R., MORGAN, E.D., CRUZ-LANDIN, C.D. Comparative study of the cuticular hydrocarbon composition of Melipona bicolor Lepeletier, 1836 (Hymenoptera, Meliponini) workers and queens. Genet Mol Res. Vol. 2, p. 191-199, 2003.
BACCI, L., PEREIRA, E.J.G., FERNANDES, F.L., PICANÇO, M.C., CRESPO, A.L.B., CAMPOS, M.R. Seletividade Fisiológica de Inseticidas a Vespas Predadoras (Hymenoptera: Vespidae) de Leucoptera coffeella (Lepidoptera: Lyonetiidae). BioAssay. Vol. 1, p. 1-7, 2006.
BAPTISTA, A.P.M., CARVALHO, G.A., CARVALHO, S.M., CARVALHO, C.F., BUENO FILHO, J.S.S. Toxicidade de produtos fitossanitários utilizados em citros para Apis mellifera. Cienc. Rural, Vol. 39, p. 955-961, 2009.
BARBOZA, H.T.G., NASCIMENTO, X.P.R., FREITAS-SILVA, O., SOARES, A.G., DACOSTA, J.B.N. Compostos Organofosforados e seu Papel na Agricultura. Ver Virtual Quim. Vol. 10, p. 172-193, 2018.
BASTOS, L.H.P., GOUVÊA, A.V., ORTIZ, N.D., CARDOSO, M.H.W.M., JACOB, S.C., NÓBREGA, A.W. Monitoramento de resÃduos de agrotóxicos da classe do organofosforados por CG-DFC em amostras de leite fluÃdo e em pó. QuÃmica Nova. Vol. 38, p. 178-184, 2015.
BATISTA, G.C., AMARAL, E., PASSARELA-NETO, A. Toxicidade de alguns inseticidas e acaricidas para operárias de Apis mellifera ligustica L. e Apis mellifera adansonii L. (Hymenoptera: Apidae). An Soc. Entomol. do Brasil. Vol. 4, p. 73-77, 1975.
CAMARGO, J.M.F., PEDRO, S.R.M. Meliponini Lepeletier, 1836. In: MOURE, J.S., URBAN, D., MELO, G.A.R. (Orgs.), Catalogue of bees (Hymenoptera, Apoidea) in the Neotropical Region. 2013. http://www. moure.cria.org.br/catalogue.
CLAUDIANOS, C., RANSON, H., JOHNSON, R.M., BISWAS, S., SCHULER, M.A., BERENBAUM, M, R, FEYEREISEN, R., OAKESHOTT, J.G. A deficit of detoxification enzymes: pesticide sensitivity and environmental response in the honeybee. Insect Mol. Biol. Vol. 15, p. 615–636, 2006.
COUTINHO, C.F.B., TANIMOTO, S.T., GALLI, A., GARBELLINI, G.S., TAKAYAMA, M., AMARAL, R.B., MAZO, L.H., AVACA, L.A., MACHADO, S.A.S. Pesticidas: mecanismos de ação, degradação e toxidez. Ecotoxicol. e Meio Amb. Vol. 15, p. 65-72, 2005.
DESNEUX, N., DECOURTYE, A., DELPUECH, J.M. The sublethal effects of pesticides on beneficial aethropods. An. Rev. of Entomol. Vol. 52, p. 81-106, 2007.
DMITRYJUK, M., ZOTTOWSKA, K., FRACZEK, R., LIPINSKI, Z. Esterases of Varroa destructor (Acari: Varroidae), parasitic mite of the honeybee. Exp. Appl. Acarol. Vol. 62, p. 499–510, 2014.
DORNELES, A.L., SOUZA-ROSA, A., BLOCHETEIN, B. Toxicity of organophosphorus pesticides to the stingless bees Scaptotrigona bipunctata and Tetragonisca fiebrigi. Apidol. Vol. 48, p. 612-620, 2017.
ELHANANY, E., ORDENTLICH, A., DGANY, O., KAPLAN, D., SEGALL, Y., BARAK, R., VELLAN, B., SHAFFERMAN, A. Resolving pathways of interaction of covalent inhibitors with the active site of acetylcholinesterases: MALDI-TOF/MS analysis of various nerve agent phosphyl adducts. Chen. Res. Toxicol. Vol. 14, p. 912-918, 2001.
FALCO, J.R.P., HASHIMOTO, J.H., FERMINO, F., TOLEDO, V.A.A. Toxicity of thiamethoxam, behavioral effects and alterations in chromatin of Apis mellifera L, 1758 (Hymenoptera; Apidae). Res. J. Agric. Biol. Sci. Vol. 6, p. 823–828, 2010.
GAJGER, I.T., SAKAC, M., & GREGORC, A. Impact of thiamethoxam on honeybee queen (Apis mellifera carnica) reproductive morphology and physiology. Bull. Environ. Contam. Toxicol. Vol. 99, p. 297–302, 2017.
HASHIMOTO, J.H., RUVOLO-TAKASUSUKI, M.C.C., TOLEDO, V.A.A. Evaluation of the use of the inhibition esterases activity on Apis mellifera as bioindicators of insecticide thiamethoxam pesticide residues. Sociobiol. Vol. 42, p. 693–699, 2003.
HEALY, M.J., DUMANCIC, M.M., OAKESHOTT, J.G. Biochemical and physiological studies of soluble esterases from Drosophila melanogaster. Biochem. Genet. Vol. 29, p. 365-387, 1991.
LEONHARDT, S.D., WALLACE, H.M., BLUTHGEN, N., WENZEL, F. Potential role of environmentally derived cuticular compounds in stingless bees. Chemoecology. Vol. 25, p. 159-167, 2015.
MACIEIRA, O.J.D., HEBLING-BERALDO, M.J.A. Laboratory toxicity of insecticides to workers of Trigona spinipes (F., 1793) (Hymenoptera – Apidae). J. Apic. Res. Vol. 28, p. 3-6, 1989.
MAPA – MINISTÉRIO DA AGRICULTURA, PECUÃRIA E ABASTECIMENTO. Agrofit, Sistemas de Agrotóxicos Fitossanitário. 2017. http://agrofit.agricultura.gov.br/agrofit_cons/principal_agrofit_cons.
MONTELLA, I.R., SCHAMA, R., VALLE, D. The classification of esterases: an important gene family involved in insecticide resistance –a review. Mem. Inst. Oswaldo Cruz. Vol. 107, p. 437–449, 2012.
MORAES, S.S., BATISTA, A.R.L., VIANA, B.F. Avaliação da toxicidade aguda (DL50 e CL50) de inseticidas para Scaptotrigona tubiba (Smith) (Hymenoptera: Apidae): Via de contato. An. Soc. Entomol. Bras. Vol. 29, p. 31-37, 2000.
MOREIRA, D.R., SINÓPOLIS-GIGLIOLLI, A.A., FALCO, J.R.P., JULIO, A.H.F., VOLNITEM, E.A., CHAGAS, F.D., TOLEDO, V.A.A., RUVOLO-TAKASUSUKI, M.C.C. Toxicity and effects of the neonicotinoid thiamethoxam on Scaptotrigona bipunctata Lepeletier, 1836 (Hymenoptera: Apidae). Environ. Toxicol. Vol. 33, p. 1–13, 2018.
OLIVEIRA, R.A., ROAT, T.C., CARVALHO, S.M., MALASPINA, O. Side-effects of thiamethoxam on the brain and midgut of the africanized honeybee Apis mellifera (Hymenopptera: Apidae). Environ. Toxicol. Vol. 29, p. 1122–1133, 2014.
SANCHEZ-BAYO, F., GOKA, K. Pesticide residues and bees – A risk assessment. PLoS ONE. Vol. 9, p. 1-16, 2014.
SHIN, D., SMARTT, C.T. Assessment of esterase gene expression as arisk marker for insecticide resistance in Florida Culex nigripalpus (Diptera: Culicidae). J. Vector Ecol. Vol. 41, p. 63–71, 2016.
SILVA, I.P., OLIVEIRA, F.A.S., PEDROZA, H.P., GADELHA, I.C.N., MELO, M.M., SOTO-BLANCO, B. Pesticide exposure of honeybees (Apis mellifera) pollinating melon crops. Apidol. Vol. 46, p. 703-715, 2012.
SANTOS, C.F., ACOSTA, A.L., DORNELES, A.L., SANTOS, P.D.S., BLOCHETEIN, B. Queens become workers: pesticides alter caste differentiation in bees. Scient. Report. Vol. 6, p. 1-9, 2016.
SLAA, E.J., CHAVES, L.A.S., MALAGODI-BRAGA, K.S., HOFSTEDE, F.E. Stingless bees in applied pollination: practice and perspectives. Apidol. Vol. 37, p. 293–315, 2006.
VENTURIERI, G.C., ALVES, D.A., VILLAS-BÔAS, J.K., CARVALHO, C.A., MENEZES, C. et al. Meliponicultura no Brasil: Situação atual e perspectivas futuras para o uso na polinização. In: IMPERATRIZ-FONSECA, V.L., CANHOS, D.A.L., SARAIVA, A.M. Polinizadores no Brasil: contribuição e perspectivas para a biodiversidade, uso sustentável, conservação e serviços ambientais. EDUSP, São Paulo. p. 227–255, 2011.
WITTER, S., NUNES-SILVA, P. Manual de boas práticas para o manejo e conservação de abelhas nativas (meliponÃneos). Porto Alegre, Fundação Zoobotânica do Rio Grande do Sul. 144 p. 2014.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
A revista se reserva o direito de fazer alterações nas regras originais, na ortografia e na ordem gramatical, a fim de manter o idioma de culto padrão, respeitando, no entanto, o estilo dos autores. Os artigos publicados são de propriedade da revista Scientific Electronic Archives, tornando-se sua reimpressão total ou parcial, sujeitos à autorização expressa da direção da revista. A fonte original da publicação deve ser mantida. Os originais não serão devolvidos aos autores. As opiniões expressas pelos autores dos artigos são de sua exclusiva responsabilidade.
The journal reserves the right to make changes to the original rules, spelling and grammatical order, in order to keep the language of worship default, respecting, however, the style of the authors. Articles published are the property of Scientific Electronic Archives magazine, becoming its total or partial reprint, subject to the express authorization of the direction of the journal. The original source of publication should be retained. The originals will not be returned to the authors. Opinions expressed by authors of articles are solely your responsibility.
This journal uses the License Creative Commons Atribuição 4.0 Internacional.