Comparison between maceration and ultrasound-assisted extraction of white bracts with flowers of Bougainvillea spectabilis Willd.


  • B. W. Debiasi Universidade Federal de Mato Grosso campus Sinop
  • P. G. R. S. Rodrigues Federal University of Mato Grosso, Campus Sinop
  • M. P. R. Torres Federal University of Mato Grosso, Campus Sinop
  • C. Bonacorsi Federal University of Mato Grosso, Campus Sinop
  • C. R. Andrighetti Federal University of Mato Grosso, Campus Sinop
  • E. B. Ribeiro Federal University of Mato Grosso, Campus Sinop
  • D. M. S. Valladão Federal University of Mato Grosso, Campus Sinop



Extraction methods, phenols, fatty acids, gas chromatography


Obtaining bioactive compounds depends on the extraction method, with maceration being the most well-known. This method is commonly used with ethanol as an extractor solvent and requires a long period to complete the process. Alternative methods, such as ultrasound-assisted extraction, have been applied to optimize these processes. In this context, to evaluate the effect of extraction time, the ultrasound-assisted and the maceration extraction methods were performed on white bracts and flowers from Bougainvillea spectabilis Willd. (Nyctaginaceae). The antioxidant activities and fatty acid content of the different extracts were determined. The amount of phenolic compounds obtained for the maceration technique was greater, however, in the evaluation of the potential antioxidant activity by the DPPH and ABTS+ free radicals scavenging, no significant difference was verified when compared to the extraction by ultrasound for 30 minutes. In the determination of fatty acids by gas chromatography coupled with mass spectrometer it was found that the highest contents were of palmitic, linoleic, and stearic acids for both extraction methods. The 30 minutes of extraction by ultrasound proved to be the time necessary to quickly and efficiently extract the bioactive compounds from B. spectabilis. Thus, the use of ultrasound-assisted extraction, when compared to the maceration method, is an alternative for obtaining plant extracts in a short time, reducing the instability that some compounds


ABARCA-VARGAS, R., PETRICEVICH, V.L. Bougainvillea genus: A review on phytochemistry, pharmacology, and toxicology. Evidence-based Complementary and Alternative Medicine. Vol. 2018, 2018.

AL-SNAFI, A.E. Antibacterial effect of the phenolic extract of Alhagi maurorum IOSR Journal of Pharmac. Vol. 9, n. 8, p. 07-14, 2019.

ALI, M.S., IBRAHIM, S.A., AHMED, F., PERVEZ, M.K. Color versus bioactivity in the flowers of Bougainvillea spectabilis (Nyctaginaceae). Natural Product Research. Vol. 19, n. 1, p. 1-5, 2005.

ANNEGOWDA, H.V., BHAT, R., MIN-TZE, L., KARIM, A.A., MANSOR, S.M. Influence of sonication treatments and extraction solvents on the phenolics and antioxidants in star fruits. Journal of Food Science and Technology. Vol. 49, n. 4, p. 510-514, 2012.

BHAT, M., KOTHIWALE, S.K., TIRMALE, A.R., BHARGAVA, S.Y., JOSHI, B.N. Antidiabetic properties of Azardiracta indica and Bougainvillea spectabilis: In vivo studies in murine diabetes model. Evidence-based Complementary and Alternative Medicine. 2009.

CARVALHO, M.T., BERGAMASCO, R., GOMES, R.G. Métodos de extração de compostos bioativos: aproveitamento de subprodutos na agroindústria. Revista UNINGÁ Review, v. 33, n. 1, p. 66-84, 2018.

CARVALHO, P. DE O., CAMPOS, P.R.B., NOFFS, M.D.A., OLIVEIRA, J.G.D., SHIMIZU, M.T., SILVA, D.M.D. Application of microbial lipases to concentrate polyunsaturated fatty acids. Quimica Nova, v. 26, n. 1, p. 75-80, 2003.

CÉSPEDES, C.L., EL-HAFIDI, M., PAVON, N., ALARCON, J. Antioxidant and cardioprotective activities of phenolic extracts from fruits of Chilean blackberry Aristotelia chilensis (Elaeocarpaceae), Maqui. Food Chemistry. Vol.107, n. 2, p. 820-829, 2008.

CHAIRES-MARTÍNEZ, L., MONROY-REYES, E., BAUTISTA-BRINGAS, A., JIMENEZ-AVALOS, H.A., SEPULVEDA-JIMENEZ, G. Determination of Radical Scavenging Activity of Hydroalcoholic and Aqueous Extracts from Bauhinia divaricata and Bougainvillea spectabilis Using the DPPH Assay. Pharmacognosy Research. Vol. 1, n.5, p.238-244, 2009.

CHOI, H.G., LEE, H.D., KIM, S.H., NA, M.K., KIM, J.A., LEE, S.H. Phenolic Glycosides from Lindera obtusiloba and their Anti-allergic Inflammatory Activities. Natural Product Communications. Vol.8, n. 2, p. 181-182, 2013.

DONG, J., LIU, Y., LIANG, Z., WANG, W. Investigation on ultrasound-assisted extraction of salvianolic acid B from Salvia miltiorrhiza root. Ultrasonics Sonochemistry. Vol. 17, n. 1, p. 61-65, 2010.

FARAG, R.S., BADEI, A.Z.M.A., HEWEDI, F.M., EL‐BAROTY, G.S.A. Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media. Journal of the American Oil Chemists Society. Vol. 66, n. 6, p. 792-799, 1989.

FAWAD, S.A., KHALID, N., ASGHAR, W., SULERIA, H.A.R. In vitro comparative study of Bougainvillea spectabilis “stand” leaves and Bougainvillea variegata leaves in terms of phytochemicals and antimicrobial activity. Chinese Journal of Natural Medicines. Vol. 10, n.6, p. 441-447, 2012.

FERNÁNDEZ-BARBERO, G., PINEDO, C., ESPADA-BELLIDO, E., FERREIRO-GONZÁLEZ, M., CARRERA, C., PALMA, M., GARCIA-BARROSO, C. Optimization of ultrasound-assisted extraction of bioactive compounds from jabuticaba (Myrciaria cauliflora) fruit through a box-behnken experimental design. Food Science and Technology. Vol. 39, n. 4, p.1018-1029, 2019.

GOLTZ, C., ÁVILA, S., BARBIERI, J.B., IGARASHI-MAFRA, L., MAFRA, M.R. Ultrasound-assisted extraction of phenolic compounds from Macela (Achyrolcine satureioides) extracts. Industrial Crops and Products. Vol. 115, p. 227-234, 2018.

GONZÁLEZ-CENTENO, M.R., COMAS-SERRA, F., FEMENIA, A., ROSSELLÓ, C., SIMAL, S. Effect of power ultrasound application on aqueous extraction of phenolic compounds and antioxidant capacity from grape pomace (Vitis vinifera L.): Experimental kinetics and modeling. Ultrasonics Sonochemistry. Vol. 22, p. 506-514, 2014.

GUPTA, M. Practical Guide to Vegetable Oil Processing. 2nd. ed. Lynnwood, TX, United States: Elsevier, 2017.

IPNI. International plant names index. Bougainvillea spectabilis Willd. Disponível em: <>.

JACQUES, R.A., FREITAS, L.S., PÉREZ, V.F., DARIVA, C., DE OLIVEIRA, A.P., DE OLIVEIRA, J.V., CARAMÃO, E.B. The use of ultrasound in the extraction of Ilex paraguariensis leaves: A comparison with maceration. Ultrasonics Sonochemistry. Vol. 14, n. 1, p. 6-12, 2007.

JAWLA, S., KUMAR, Y., KHAN, M.S.Y. Hypoglycemic activity of Bougainvillea spectabilis stem bark in normal and alloxan-induced diabetic rats. Asian Pacific Journal of Tropical Biomedicine. Vol. 2, n. 2, p. S919–S923, 2012.

JHAM, G. N.; TELES, F. F. F.; CAMPOS, L. G. Use of aqueous HCI/MeOH as esterification reagent for analysis of fatty acids derived from soybean lipids. Journal of the American Oil Chemists Societ. Vol. 59, n.3, p.132-133, 1982.

KHOEI, M., CHEKIN, F. The ultrasound-assisted aqueous extraction of rice bran oil. Food Chemistry. Vol. 194, p. 503–507, 2016.

MARTÍNEZ-YUSTA, A., GUILLÉN, M.D. Deep-frying food in extra virgin olive oil: A study by 1H nuclear magnetic resonance of the influence of food nature on the evolving composition of the frying medium. Food Chemistry. Vol. 150, p. 429-437, 2014.

MELECCHI, M.I.S., PÉRES, V.F., DARIVA, C., ZINI, C.A., ABAD, F.C., MARTINEZ, M.M., CARAMÃO, E.B. Optimization of the sonication extraction method of Hibiscus tiliaceus L. flowers. Ultrasonics Sonochemistry. Vol.13, n.3, p.242-250, 2006.

MEREGALLI, M.M., PUTON, B.M.S., CAMERA, F.D.M., AMARAL, A.U., ZENI, J., CANSIAN, R.L., MIGNONI, M.L, BACKES, G.T. Conventional and ultrasound-assisted methods for extraction of bioactive compounds from red araçá peel (Psidium cattleianum Sabine). Arabian Journal of Chemistry. Vol. 13, n. 6, p. 5800-5809, 2020.

MURRAY, R.K., GRANNER, D.K., MAYES, P.A., RODWELL, V.W. Harper’s Illustrated Biochemistry. 26 ed. Lange Medical Publications, 2003.

NADEEM, M., UBAID, N., QURESHI, T.M., MUNIR, M., MEHMOOD, A. Effect of ultrasound and chemical treatment on total phenol, flavonoids and antioxidant properties on carrot-grape juice blend during storage. Ultrasonics Sonochemistry. Vol. 45, p. 1-6, 2018.

NEVES, L.C., ALENCAR, S.M.DE; CARPES, S.T. Determinação da atividade antioxidante e do teor de compostos fenólicos e flavonoides totais em amostras de pólen apícola de Apis mellifera. Brazilian Journal of Food Technology. Vol.7, p.107-110, 2009.

PHAM, D.C., NGUYEN, H.C., NGUYEN, T.H.L., HO, H.L., TRINH, T.K., RIYAPHAN, J., WENG, C.F. Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities. BioMed Research International. Vol.2020, 2020.

PIRES, J., TORRES, P.B., SANTOS, D.Y.A.C., CHOW, F. Ensaio em microplaca do potencial antioxidante através do método de sequestro do radical livre DPPH para extratos de algas. Instituto de Biociências, Universidade de São Paulo, p. 6, 2017.

RICE, T.W., WHEELER, A.P., THOMPSON, B.T., DEBOISBLANC, B.P., STEINGRUB, J., ROCK, P. Enteral omega-3 fatty acid, γ-linolenic acid, and antioxidant supplementation in acute lung injury. JAMA - Journal of the American Medical Association. Vol. 306, n. 14, p. 1574-1581, 2011.

RICHARD, D., KEFI, K., BARBE, U., BAUSERO, P., VISIOLI, F. Polyunsaturated fatty acids as antioxidants. Pharmacological Research. Vol. 57, n. 6, p. 451-455, 2008.

RODRÍGUEZ-PÉREZ, C., QUIRANTES-PINÉ, R., FERNÁNDEZ-GUTIÉRREZ, A., SEGURA-CARRETERO, A. Optimization of extraction method to obtain a phenolic compounds-rich extract from Moringa oleifera Lam leaves. Industrial Crops and Products. Vol.66, p.246-254, 2015.

ROESLER, R., MALTA, L.G., CARRASCO, L.C., HOLANDA, R.B., SOUSA, C.A.S., PASTORE, G.M. Atividade antioxidante de frutas do cerrado. Ciencia e Tecnologia de Alimentos. Vol.27, n.1, p. 53-60, 2007.

RUFINO, M. DO S.M., ALVES, R.E., DE BRITO, E.S., DE MORAIS, S.M., SAMPAIO, C.D.G., PÉREZ-JIMENEZ, J., SAURA-CALIXTO, F.D. Metodologia Científica: Determinação da Atividade Antioxidante Total em Frutas pela Captura do Radical Livre DPPH. Comunicado Técnico On-line nº127. Fortaleza: Embrapa Agroindústria Tropical, 2007

RUFINO, M. DO S.M., ALVES, R.E., DE BRITO, E.S., DE MORAIS, S.M., SAMPAIO, C.D.G., PÉREZ-JIMENEZ, J., SAURA-CALIXTO, F.D. Metodologia Científica: Determinação da Atividade Antioxidante Total em Frutas pela Captura do Radical Livre ABTS. Comunicado Técnico On-line nº128. Fortaleza: Embrapa Agroindústria Tropical, 2007

SAFDAR, M.N., KAUSAR, T., JABBAR, S., MUMTAZ, A., AHAD, K., SADDOZAI, A.A. Extraction and quantification of polyphenols from kinnow (Citrus reticulate L.) peel using ultrasound and maceration techniques. Journal of Food and Drug Analysis. Vol. 25, n. 3, p. 488-500, 2017.

ŞAHIN, S.; ŞAMLI, R. Optimization of olive leaf extract obtained by ultrasound-assisted extraction with response surface methodology. Ultrasonics Sonochemistry. Vol. 20, n. 1, p. 595-602, 2013.

SALEEM, H., HTAR, T.T., NAIDU, R., ZENGIN, G., AHMAD, I., AHEMAD, N. Phytochemical profiling, antioxidant, enzyme inhibition and cytotoxic potential of Bougainvillea glabra flowers. Natural Product Research. Vol. 34, n. 18, p. 2602-2606, 2019.

ŠAMEC, D., GRUZ, J., STRNAD, M., KREMER, D., KOSALEC, I., GRUBEŠIĆ, R. J., KARLOVIĆD, K., PILJAC-ŽEGARAC, J. Antioxidant and antimicrobial properties of Teucrium arduini L. (Lamiaceae) flower and leaf infusions (Teucrium arduini L. antioxidant capacity). Food and Chemical Toxicology. Vol.48, n. 1, p. 113-119, jan. 2010.

SANTI, M.M., SANCHES, F.S., SILVA, J.F.M., SANTOS, P.M.L. Determinação do perfil fitoquímico de extrato com atividade antioxidante da espécie medicinal Cordia verbenacea DC. por HPLC-DAD. Revista Brasileira de Plantas Medicinais. Vol. 16, n. 2, p. 256-261, 2014.

SILVA, N.L., BATISTA, M.R.F., SANTOS, T.B.A., FERNANDO, W.L., AMARAL, F.R. Determinação da atividade antioxidante e teor de flavonoides totais equivalentes em quercetina em folhas de Cymbopogon citratus (d.c.) stapf e Melissa officinalis lam. Conexão Ciência (Online). Vol. 12, n. 1, p. 46–53, 2017.

SIMÕES, C.M.O., SCHENKEL, E.P., GOSMANN, G., MELLO, J.C.P., MENTZ, L.A., PETROVICK, P.R. Farmacognosia: da planta ao medicamento. 6. ed. Porto Alegre: Editora da UFSC, 2010.

SOQUETTA, M.B., TERRA, L. DE M., BASTOS, C.P. Green technologies for the extraction of bioactive compounds in fruits and vegetables. CYTA - Journal of Food. Vol. 16, n. 1, p. 400-412, 2018.

SWAMY, K.M., LOKESH, P., RASHMI, W., VIJAY, R., SSN, K. Phytochemical screening and in vitro antimicrobial activity of Bougainvillea spectabilis flower extracts. International Journal of Phytomedicine 4. Vol. 4, n. 3, p. 375–379, 2012.

TAN, C.X., HEAN, C.G., HAMZAH, H., GHAZALI, H.M. Optimization of ultrasound-assisted aqueous extraction to produce virgin avocado oil with low free fatty acids. Journal of Food Process Engineering. Vol. 41, n. 2, p. 1-9, 2009.

TIAN, Y., XU, Z., ZHENG, B., LO, Y.M. Optimization of ultrasonic-assisted extraction of pomegranate (Punica granatum L.) seed oil. Ultrasonics Sonochemistry. Vol. 20, n. 1, p. 202-208, 2013.

WALSH, D.J., LIVINGHOUSE, T., GOERES, D.M., METTLER, M., STEWART, P.S. Antimicrobial Activity of Naturally Occurring Phenols and Derivatives Against Biofilm and Planktonic Bacteria. Frontiers in Chemistry. Vol. 7, p. 1-13, 2019.

WANG, J., SUN, B., CAO, Y., TIAN, Y., LI, X. Optimisation of ultrasound-assisted extraction of phenolic compounds from wheat bran. Food Chemistry. Vol. 106, n. 2, p. 804-810, 2008.

WANG, Z.J., LIANG, C.L., LI, G. M., YU, C.Y., Yin, M. Stearic acid protects primary cultured cortical neurons against oxidative stress. Acta Pharmacologica Sinica. Vol. 28, n. 3, p. 315-326, 2007.

WENG, J.R., LIN, C.S., LAI, H.C., LIN, Y.P., WANG, C.Y., TSAI, Y.C., WU, K.C., HUANG, S.H., LIN, C. W. Antiviral activity of Sambucus Formosana nakai ethanol extract and related phenolic acid constituents against human coronavirus NL63. Virus Research. Vol. 273, p. 197767, 2019.

ZHAO, S.; KWOK, K. C.; LIANG, H. Investigation on ultrasound assisted extraction of saikosaponins from Radix Bupleuri. Separation and Purification Technology. Vol. 55, n. 3, p. 307-312, 2007




Como Citar

Debiasi, B. W., Rodrigues, P. G. R. S., Torres, M. P. R., Bonacorsi, C., Andrighetti, C. R., Ribeiro, E. B., & Valladão, D. M. S. (2021). Comparison between maceration and ultrasound-assisted extraction of white bracts with flowers of Bougainvillea spectabilis Willd. Scientific Electronic Archives, 14(2), 47–55.



Biological Science

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