Influence of the estrous cycle on prolactin secretion induced by acute stress in nulliparous and primiparous rats


  • F. M. Silva
  • T. V. Souza
  • R. C. A. Berber
  • E. E. T. S. Hucke



Acute Stress, Estrous cycle, Prolactin


Prolactin is classically considered a hormone related to the development of the mammary glands during pregnancy and the production of milk in the postpartum. The release of this pituitary hormone can be interfered by stress, resulting in increased production. Physiological factors such as reproductive experience, sum of pregnancy, childbirth and lactation, and the phase of the estrous cycle has also an action on the secretion of prolactin, reducing their serum concentration. In previous studies, we observed that reproductive experience is able to modulate the release of prolactin in response to acute stress and that this is related to the type of stress applied in rats. In this context, the aim of this work was to study the influence of the estrous cycle on prolactin serum concentrations during the first hour of induction of acute stress in nulliparous and primiparous rats. The results indicated that reproductive experience modulates the secretion of prolactin released in response to acute stress and that this is dependent on the stage of the estrous cycle


ANSELMO-FRANCI, J. A.; SZAWKA, R. E. Controle Neuroendócrino da Reprodução Feminina. In: Neuroendocrinologia básica e aplicada. Rio de Janeiro: Guanabara Koogan, p. 318-340, 2005.

BEN-JONATHAN, N. Editorial: dopamine and prolactin – an imperfect duo in circadian rhytimicity. Endocrinology, n. 137, p. 3619-3620, 1996.

BOCHINI, J. C.; Efeito da adrenalectomia sobre a secreção de prolactina em ratas nulíparas e primíparas. Laboratório de Fisiologia e Farmacologia, Faculdade de Medicina Veterinária, UNIFEOB, SP. Fapesp Proc. 03/01887-7, 2004.

BRIDGES, R. S.; DIBIASE, R.; LOUNDES, D. D.; DOHERTY, P. Prolactin stimulation of maternal behavior in female rats. Science, n. 227, p. 782-784, 1985.

BRIDGES, R. S.; FELICIO, L. F.; PELLERIN, L. J.; STUER, A. M.; MANN, P. E. Prior parity reduces post-coital diurnal and nocturnal prolactin surges in rats. Life Science, n. 53, p. 439-445, 1993.

BYRNES, E. M.; BRIDGES, R. S. Reproductive experience alters anxiety-like behavior in the female rat. Hormones and Behavior, v. 50, p. 70-76, 2006a.

BYRNES, E. M.; BRIDGES, R. S. Reproductive experience reduces the sedative, but not anxiolytic effects of diazepam. Psychoneuroendocrinology, v. 31, p. 988-996, 2006b.

CRUZ-CASALLAS, P.E.; NASELLO, A.G.; HUCKE, E.E.T.S.; FELICIO, L.F. Dual modulation of male sexual behavior in rats by central prolactin: relationship with in vivo striatal dopaminergic and serotoninergic activity. Psychoneuroendocrinology, v.24, p.681 - 693, 1999.

GALA, R.R.; HAISENLEDER, D.J. Restraint stress decreases afternoon plasma prolactina levels in females rats. Influences of neural antagonists and agonists on restraint-induced changes in plasma prolactina and corticosterone. Neuroendocrinology, n. 43, p. 115-123, 1986.

HORVÃTH, K. M.; BÃNKY, Z.; TÓTH, B. T.; HALÃSZ, B.;NAGY, G. M. Effect of adrenalectomy and dexamethasone treatment on prolactin secretion of lactating rats. Brain Research Bulletin, v.56, n. 6, p. 589-592, 2001.


Reproductive Experience reduces striatal dopaminergic responses in freely moving female rats. Neuroreport, v.9, p.3589 - 3593, 1998.

HUCKE, E.E.T.S.; CRUZ-CASALLAS, P.E.; SIDER, L.H.; FELICIO, L.F. Reproductive Experience modulates dopamine-related responses. Pharmacology Biochemistry and Behavior, v.68, p.575 - 582, 2001.

HUCKE, E.E.T.S; FELICIO, L.F. Effects of prolactin on in vivo striatal monoaminergic activity are modulated by a previous reproductive experience. Brazilian Journal of Veterinary Research and Animal Science, v. 44 (supl.), p. 71-80, 2008.

KINSLEY, C. H.; BRIDGES, R. S. Parity-associated reduction in behavioral sensitivity to opiates. Biology of Reproduction, n. 39, p. 270-278, 1988.

LEITE, C. B.; Efeito do estresse agudo e prolongado sobre a secreção de prolactina em ratas nulíparas e primíparas. Laboratório de Fisiologia e Farmacologia, Faculdade de Medicina Veterinária, UNIFEOB, SP. Fapesp: Proc. 04/15197-5R, 2007.

LIU, B.; ARBOGAST, L.A. Progesterone decreases tyrosine hydroxylase phosphorylation state and increases protein phosphatase 2a activity in the stalk-median eminence on proestrous afternoon. J Endocrinol. 204(2):209-19, 2010.

MANN, P. E.; BRIDGES, R. S. Neural and endocrine sensitivities to opioids decline as a function of multiparity in the rat. Brain Research, n. 580. p. 241-248, 1992.

MUSEY, V. C.; COLLINS, D. C.; BROGAN, D. R.; SANTOS, V. R.; MUSEY, P. I., MARTINO-SALTZMAN, D.; PREEDY, J. R. K. Long term effects of the first pregnancy on the hormonal environment: estrogens and androgens. Journal of Clinical Endocrinology and Metabolism, v. 64, p. 111-118, 1987a.

MUSEY, V. C., COLLINS, D. C.; MUSEY, P. I.; MARTINO-SALTZMAN, D.; PREEDY, J. R. K. Long-term effect of a first pregnancy on the secretion of prolactin. New England Journal of Medicin, v. 316, p. 229-234, 1987b.


PARKER, V.J.; DOUGLAS, A.J. Stress in early pregnancy: maternal neuro-endocrine-immune responses and effects. J. Reprod. Immunol. 85(1):86-92, 2010.

SIDER, L.H.; HUCKE, E.E.T.S.; FLORIO, J.C.; FELICIO, L.F. Influence of time of day on hypothalamic monoaminergic activity in early pregnancy: effect of a previous reproductive experience. Psychoneuroendocrinology, v.28, p.195 - 206, 2003.

SIDER, L.H.; HUCKE, E.E.T.S.; FELICIO, L.F. Modulation of post-coital dopaminergic tone by prior parity. Brazilian Archives of Biology and Technology, vol.53, no.3, p.615-622, 2010.

SILVA, E.C.; CASTRO, L. Regulação da secreção de prolactina. In: Neuroendocrinologia básica e aplicada. Antunes-Rodrigues, J.; Moreira, A.C.; Elias, L.L.K.; Castro, M. (eds.). Guanabara Koogan: Rio de Janeiro, Brasil, p. 341-354, 2005.

SMITH, W.W.; GALA, R.R. Influence of restrain on plasma prolactin and corticosterone in female rats. Journal of Endocrinology, n. 74, p. 303-314, 1977.

STERN, J. M.; VOOGT, J. L. Comparison of plasma corticosterone and prolactin levels in cycling and lactating rats. Neuroendocrinology, n. 13, p. 173-181, 1974.

SZAWKA, R. E.; FRANCI, C. R.; ANSELMO-FRANCI, J. A. Noradrenaline release in the medial preoptic área during the rat oestrous cycle: temporal relationship with plasma secretory surges of prolactin and luteinising hormone. Journal of Neuroendocrinology, n. 19, p. 374-382, May, 2007a.

SZAWKA, R.E.; RODOVALHO, G.V.; HELENA, C.V.; FRANCI, C.R.; ANSELMO-FRANCI, J.A. Prolactin secretory surge during estrus coincides with increased dopamine activity in the hypothalamus and preoptic area and is not altered by ovariectomy on proestrus. Brain Research Bulletin, 15, 73(1-3):127-34, 2007b.

UZUELLI, F. H. P.; Metabolismo de radicais livres durante o ciclo estral de ratas. Dissertação (Mestrado em Ciências da Saúde). Universidade de Brasília, Brasília, 2006.

THRIVIKRAMAN, K. V.; HUOT, R. L. ; PLOTSKY, P. M. Jugular vein catheterization for repeated blood sampling in the unrestrained conscious rat. Brain Research Protocols. v. 10, p. 84–94, 2002.

YOKOYAMA, K.; HAYASHI, M.; MOGI, C.; SASAKAWA, Y.; WATANABE, G.; TAYA, K.; DEVNATH, S.; INOUE, K. Dose-dependent effects of a glucocorticoid on prolactin production. Endocr. J. 55(2):405-14, 2008.

WARTELLA, J.; AMORY,E.; MACBETH, A. H.; STEVENS, L.; LAMBERT, K. G.; KINSLEY, C. H.; Single or multiple reproductive experiences attenuate neurobehavioral stress and fear responses in the female rat. Physiology & Behavior, v. 79, p. 373-381, 2003.




Como Citar

Silva, F. M., Souza, T. V., Berber, R. C. A., & Hucke, E. E. T. S. (2017). Influence of the estrous cycle on prolactin secretion induced by acute stress in nulliparous and primiparous rats. Scientific Electronic Archives, 10(4), 32–39.



Ciências Biológicas

Artigos mais lidos pelo mesmo(s) autor(es)

<< < 1 2 3 > >>