Evaluation of the in vitro cytotoxic effects of carvoeiro leaf extracts [Callisthene fasciculata (Spr.) Mart.]


  • L. Castoldi Universidade Federal de Mato Grosso
  • T. O. Kelly Federal University of Mato Grosso - UFMT
  • L. R. Albiero Federal University of Mato Grosso - UFMT
  • E. F. Nery Federal University of Mato Grosso - UFMT
  • J. C. S. Dalazen Federal University of Mato Grosso - UFMT
  • D. M. S. Valladão Federal University of Mato Grosso - UFMT




Callisthene fasciculata (Spr.) Mart., carvoeiro, cytotoxicity, Walker 256 tumor, spleen cell


Callisthene fasciculata (Spr.) Mart. is a tropical tree belonging to the family Vochysiaceae and is popularly known as carvoeiro. In popular medicine, it is used for the treatment of hepatitis, stomach diseases, kidney problems, jaundice, and anemia; however, its effects have not been scientifically proven. Therefore, the objective of the present study was to conduct a preliminary study on the biological effects of carvoeiro by evaluating its in vitro cytotoxic effect in co-culture with different cell types. To prepare the extract, 70% ethanol (ratio, 1:5) was added to the dried and crushed leaves, which were incubated for 7 days in the dark. Next, the extract was filtered and evaporated in a rotavapor to reduce the volume and obtain a powder. The powder was resuspended in RPMI culture medium supplemented with 20% fetal bovine serum and added to Walker 256 tumor cells or splenic cells (rat or mice) at different concentrations (serial dilutions of 1000 μg/ml to 7.81 μg/ml). After 24 h, cytotoxicity analysis was performed by the colorimetric MTT reduction assay. The results demonstrated that the extract favored the viability of tumor and rat spleen cells at higher concentrations (Walker tumor: 1000 μg/ml, 0.685 ± 0.066, 500 μg/ml, 0.565 ± 0.067; 250 μg/ml, 0.329 ± 0.019; control, 0.168 ± 0.01; rat spleen: 1000 μg/ml, 0.334 ± 0.045; control: 0.219 ± 0.053) and showed a cytotoxic effect at lower concentrations, with the percentage of cell inhibition ranging from 7% to 19%. According to the results, we concluded that the leaf extract of Callisthene fasciculata (Spr.) Mart had a direct effect on the studied cells in vitro. This was the first study to report the biological effect of this plant; however, further study is needed to determine its effects on complex systems, such as those found in vivo.

Biografia do Autor

L. Castoldi, Universidade Federal de Mato Grosso




AKRAM, M., HAMID, A., KHALIL, A., GHAFFAR, A., TAYYABA, N., SAEED, A., ALI, M., NAVEED, A. Review on medicinal uses, pharmacological, phytochemistry and immunomodulatory activity of plants. Internacional Journal of Immunopathology and pharmacology 27: 313-319, 2014. http://journals.sagepub.com/doi/pdf/10.1177/039463201402700301.

ALBIERO, LR., NERY, EF., DALAZAN, JC., KELLY, TO., PEREIRA, L., SINHORIN, VDG., KANENO, R., CASTOLDI, L. Ethanolic Extracts of Copaifera Multijuga Inhibits the Subcutaneous Growth of Ehrlich Carcinoma in Swiss Mice. Journal of Pharmacy and Biological Sciences 11: 30-38, 2016. http://www.iosrjournals.org/iosr-jpbs/papers/Vol11issue5/Version3/G1105033038.pdf.

BONACORSI, C., FONSECA, LM., RADDI, MSG., KITAGAWA, RR., VILEGAS, W. Comparison of Brazilian Plants Used to Treat Gastritis on the Oxidative Burst of Helicobacter pylori-Stimulated Neutrophil. Evidence-Based Complementary and Alternative Medicine 2013: 1-8, 2013. file:///C:/Users/Dell/Downloads/851621.pdf.

BRASIL. Lei n° 71, de 2 de setembro de 2013. Diário Oficial da República, Brasília, DF, 2 set. 2013. Série 1, p. 5439.

CARNEIRO, M.R.B. A Flora Medicinal no centro oeste do Brasil: um estudo de caso com abordagem etnobotânica em Campo Limpo de Goiás. 242f. (Dissertação de Mestrado) – Centro Universitário de Anápolis, Anápolis, Brasil, 2009. http://www.unievangelica.edu.br/files/images/curso/mestrado.mstma/2009/marcos%20rodrigo%20%20a%20flora%20medicinal.pdf.

CASTOLDI, L., GOLIM, MA., FILHO, OG., ROMAGNOLI, GG., IBAÑEZ, OC., KANENO, R. Enhanced natural killer activity and production of pro-inflammatory cytokines in mice selected for high acute inflammatory response (AIRmax). Immunology 120: 372-379, 2006. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265892/pdf/imm0120-0372.pdf.

COLQUHOUN, A., SCHUMACHER, RI. y-Linolenic acid and eicosapentaenoic acid induce modications in mitochondrial metabolism, reactive oxygen species generation, lipid peroxidation and apoptosis in Walker 256 rat carcinosarcoma cells. Biochimica et Biophysica Acta 1533: 207-219, 2001.

CORDEIRO, TM., BORGHETTI, F., OLIVEIRA, SCC, BASTOS, IMD., SANTANA, J.M., GRELLIER, P., CHARNEAU, S. Brazilian Cerrado Qualea grandiflora Mart. Leaves Exhibit Antiplasmodial and Trypanocidal Activities In vitro. Pharmacogn Mag 13: 1-7, 2017. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701409/.

FERREIRA, PMP., LIMA, DJB., DEBIASI, BW., SOARES, BM., MACHADO, KC., NORONHA, JC., RODRIGUES, DJ., SINHORIN, AP., PESSOA, C., VIEIRA JUNIOR, GM. Antiproliferative activity of Rhinella marina and Rhaebo guttatus venom extracts from Southern Amazon. Toxicon 72: 43-51, 2013. https://ac.els-cdn.com/S0041010113002237/1-s2.0-S0041010113002237-main.pdf?_tid=3dd8f01f-ccf1-4e87-af01-118cafde8b4e&acdnat=1525269695_9bceb0ea6f8adb12e473df538dafa627.

HESS, SC., BRUM, RL., HONDA, NK., CRUZ, AB., MORETTO, E., CRUZ, RB., MESSANA, I., FERRARI, F., FILHO, VC., YUNES, RA. Antibacterial activity and phytochemical analysis of Vochysia divergens (Vochysiaceae). Journal of Ethnopharmacology 47: 97-100, 1995.

KANENO, R., FONTANARI, LM., SANTOS, SA., DI STASI, LC., RODRIGUES FILHO, E., EIRA, AF. Effects of extracts from Brazilian Sun-mushroom (Agaricus blazei) on the NK activity and lymphoproliferative responsiveness of Ehrlich tumor-bearing mice. Food and Chemical Toxicol 42: 909-916, 2004.

MARTEL, J., KO, YF., OJCIUS, DM., LU, CC., CHANG, CJ., LIN, CS., LAI, HC., YOUNG, JD. Immunomodulatory properties of plants and mushrooms. Trends in Pharmacological Sciences 38: 967 – 981, 2017.

MARTINS, MD., MARQUES, MM., BUSSADORI, SK., MESQUITA-FERRARI, RA., PAVESI, VCS., WADT, NS., FERNADES, KP. Citotoxicidade in vitro de extratos de arnica brasileira (Solidago microglossa) e arnica paulista (Porophyllum ruderale). Conscientiae Saúde 8: 99 – 104, 2009. https://www4.uninove.br/ojs/index.php/saude/article/viewFile/1457/1207.

MARTINS, PR, GAMEIRO, MC., CASTOLDI, L., ROMAGNOLI, GG., LOPES, FC., PINTO, AVFS., LOYOLA, W., KANENO, R. Polysaccharide-rich fraction of Agaricus brasiliensis enhances the candidacidal activity of murine macrophages. Memórias do Instituto Oswaldo Cruz 103: 244¬250, 2008. http://www.scielo.br/pdf/mioc/v103n3/137.pdf.

MAYWORM, MAS., BUCKERIDGE, MS., SALATINO, A. Monomer composition of polysaccharides of seed cell walls and the taxonomy of the Vochysiaceae. Phytochemistry 55: 581-587, 2000.

MAYWORM, MAS., SALATINO, A. Distribution of seed fatty acids and the taxonomy of Vochysiaceae. Biochemical Systematic and Ecology 30: 961-972, 2002.

MAYWORN, AS., BUCKERIDGE, MS., MARQUEZ, UML., SALATINO, A. Nutricional reserves of Vochysiaceae seeds: chemical diversity and potencial economic uses. Anais da Academia Brasileira de Ciências 83: 523-531, 2011. http://www.scielo.br/pdf/aabc/v83n2/v83n2a12.pdf.

MAZZOLIN, PL., KIGUTI, LRA., MAIA, EO., FERNANDES, LTL., VILEGAS, W., PUPO, AS., DI STASI, LC., HIRUMA-LIMA, CA. Antidiarrheal and intestinal anti-inflammatory activities of a methanolic extract of Qualea parviflora Mart. in experimental models. Journal of Ethnopharmacology 150: 1016-1023, 2013.

NETO, GG. Plantas do Brasil – angiospermas do Estado de Mato Grosso – Pantanal. Acta botânica brasileira 5: 25- 47, 1991. http://www.scielo.br/pdf/abb/v5n1/v5n1a03.pdf.

OLIVEIRA, LP., PINHEIRO, RC., VIEIRA, MS., PAULA, JR., BARRA, MT., VALADARES, MC. Atividade citotóxica e antiangiogênica de Punica granatum L., Punicaceae. Revista Brasileira de Farmacognosia 20: 201 – 207, 2010. http://www.scielo.br/pdf/rbfar/v20n2/a11v20n2.pdf.

ROGERO, SO., LUGÃO, AB., IKEDA, TI., CRUZ, AS. Teste in vitro de citotoxicidade: estudo comparativo entre duas metodologias. Materials Research 6: 317 – 320, 2003. http://www.scielo.br/pdf/mr/v6n3/a03v6n3.pdf.

SPINARDI-BARBISAN, ALT., KANENO, R., BARBISAN, LF., CAMARGO, JVC, RODRIGUES, MAM. Chemically induced immunotoxicity in a medium-term multiorgan bioassay for carcinogenesis with Wistar rats. Toxicology and Applied Pharmacology 194: 132– 140, 2004.

SULTANA, S., ASIF., HM., NAZAR, HMI., AKHTAR, N., REHMAN, JU., REHMAN, RU. Medicinal Plants Combating Against Cancer – a Green Anticancer Approach. Asian Pacific Journal of Cancer Prevention 15: 4385-4394, 2014. http://journal.waocp.org/article_29255_5aea359ba0421b89c9419048ae1d5ddf.pdf.




Como Citar

Castoldi, L., Kelly, T. O., Albiero, L. R., Nery, E. F., Dalazen, J. C. S., & Valladão, D. M. S. (2018). Evaluation of the in vitro cytotoxic effects of carvoeiro leaf extracts [Callisthene fasciculata (Spr.) Mart.]. Scientific Electronic Archives, 11(5), 124–129. https://doi.org/10.36560/1152018668



Biological Science

Artigos mais lidos pelo mesmo(s) autor(es)

> >>