TY - JOUR AU - Belo, A. P. M. AU - Vieira, M. C. AU - Guimarães, H. O. AU - Oliveira, T. C. AU - Mascarenhas, Y. S. AU - Souza, E. R. B. PY - 2018/04/04 Y2 - 2024/03/28 TI - Survival rates and contamination of nodes three varieties of Hancornia speciosa in different concentrations of sodium hypochlorite and immersion times JF - Scientific Electronic Archives JA - Sci. Electronic Arch VL - 11 IS - 2 SE - Ciências Agrárias DO - 10.36560/1122018419 UR - https://sea.ufr.edu.br/SEA/article/view/419 SP - 48 - 57 AB - The objective of this research was to develop a protocol for the disinfection and <em>in vitro</em> survival of explants from different varieties of <em>Hancornia speciosa </em>Gomes. Shoot tip containing nodal segments of three varieties of <em>H</em>.<em> speciosa</em>, they were collected and transported to the Tissue Culture Laboratory vegetables of Agronomy of the Federal University of Goias - UFG. In this place, the sheets were removed and subjected to aseptic nodal in 70% alcohol for 2 minutes, followed by immersion in sodium Hypochlorite of the Sodium, commercial product bleach KiJóia®  concentrations of 1.0%; 1.5% to 2.0% for 5 or 10 minutes. Infection rates were evaluated, oxidation; types of contaminating microorganisms; living shoots; shoots dead; living explants and pair of leaf number. Different varieties have spoken out about the different disinfestation and survival process. It is possible the cultivation <em>in vitro</em> explants <em>H. speciosa</em> nodal coming from the field. ER -