Integrated production systems revealing antagonistic fungi biodiversity in the tropical region

Autores

  • G. C. M Berber Federal University of Rondonópolis
  • S. M. Bonaldo
  • K. B. Carmo
  • M. Garcia
  • A. Farias Neto
  • A. Ferreira

DOI:

https://doi.org/10.36560/13620201150

Palavras-chave:

Microbial ecology, crop-livestock-forestry, Biocontrol, 18S rDNA, Plant soil

Resumo

The antagonism and diversity of fungi have been studied in several environments, including agricultural soils. Nevertheless, information regarding fungi that are able to control Fusarium sp., Rhizoctonia sp. and Sclerotium rolfsii in integrated Crop-Livestock-Forest systems soils is unknown. Ten treatments were assessed, including monoculture, integration of Crop-Livestok-Forest, fallow and native forest. During the rainy and dry season was carried out fungi colony forming units (CFU), antagonistic potential and molecular identification. The results showed that CFU were higher in the rainy season and integrated systems of production. Fungal isolates as Penicillium, Talaromyces, Eupenicillium, Trichoderma, Aspergillus, Chaetomium, Acremonium, Curvularia, Purpureocillium, Bionectria, Paecilomyces, Plectospharella, Clonostachy, Mucor, Fennellia and Metarhizium were able to control Rhizoctonia sp., Fusarium sp. and Sclerotium rolfsii. This is the first report to describe culturable fungi species from the Amazon biome that are able to control pathogens. Furthermore, we suggest that integrated production systems can be a strategy for increasing fungal biomass and the rainy and dry season can modulate the density of soil fungi also, mainly in a tropical region.

Biografia do Autor

G. C. M Berber, Federal University of Rondonópolis

Institute of Agricultural and Technological Sciences

Referências

Ahmed, A.S., Ezziyyani, C., Sánchez, C.P., Candela, M.E., 2003. Effect of chitin on biological control activity of Bacillus spp. and Trichoderma harzianum against root rot disease in pepper (Capsicum annuum) plants. Eur. J. Plant Pathol. 109, 633-637.

Alabouvette, C.; Olivain, C.; Migheli, Q.; Steinberg, C., 2009. Microbiological control of soil-borne phytopathogenic fungi with special emphasis on wilt-inducing Fusarium oxysporum. New Phytol., 184, 529–544.

Alvares, C.A.; Stape, J.L.; Sentelhas, P.C.; Gonçalves, J.L.M.; Sparovek, G., 2013. Köppen’s climate classification map for Brazil. Meteorol. Z. 22, 711-728.

Alves, R.T., Bateman, R.P., 2013. Evaluation of formulation and volume application rate on the secondary pick-up of Metarhizium acridum (Driver & Milner) Bischoff, Rehner & Humber conidia on Schistocerca gregaria (Forskål) (Orthoptera: Acrididae) BioAssay. 8, 4.

Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J., 1990. Basic local alignment search tool. J. Mol. Biol. 215, 403-410.

Alves, T.S.A., Campos, L.L., Neto, N.E., Matsuoka, M., Loureiro, M.F., 2011. Biomassa e Atividade Microbiana de Solo Sob Vegetação Nativa e Diferentes Sistemas de Manejos. Acta Scient. Agron. 33, 341-347.

Balbino, L.C., Cordeiro, L.A.M., Vanderley, O.S., Moraes, A., 2011. Evolução tecnológica e arranjos produtivos de sistemas. Pesq. Agropec. Bras. 46, 10, i-xii.

Carvalho, D.D.C., Mello, S.C.M.D., Lobo Junior, M., Geraldine, A.M., 2011. Biocontrol of seed pathogens and growth promotion of common bean seedlings by Trichoderma harzianum. Pesq. Agropec. Bras. 46, 822–828.

Cortinovis, C., Pizzo, F., Spicer, L.J., Caloni, F., 2013. Fusarium mycotoxins: Effects on reproductive function in domestic Animals – A review. Theriogenology. 80, 557–564.

Corrêa, L.E., Bettiol, W., Morandi, M.A.B., 2010. Biological control of Pythium aphanidermatum. Trop. Plant. Pathol. 35, 7.

FAO (Food and Agriculture Organization) of the United Nations, 2012. Agriculture and consumer protection department. Conservation agriculture, http://www.fao.org/nr/cgrfa/cthemes/cgrfa-micro-organisms.

Fernando, W.G.D., Nakkeeran, S., Zhang, Y., Savchuk, S., 2007. Biological control of Sclerotinia sclerotiorum (Lib.) de Bary by Pseudomonas and Bacillus species on canola petals. Crop. Protect. 26, 100–107.

Ferraz, L.D.C.L., Nasser, L.C.B., Café, F.A.C., 2011. Viabilidade de escleródios de Sclerotinia sclerotiorum e incidência de fungos antagonistas em solo de Cerrado. Summa Phytopathologica. 37, 208–210.

Figueiredo, M.B., 1967. Estudos sobre a aplicação do método de Castellani para conservação de fungos patógenos em plantas. O Biológico. 33(1), 9–13.

Gao, J.X., Liu, T., Chean, J., 2014. Insertional mutagenesis and cloning of the gene required for the biosynthesis of the non-host specific toxin in Cochliobolus lunatus that causes maize leaf spot. Phytopathol. 104, 332–339.

Geraldine, A.L., Lopes, F.A.C., Carvalho, D.D., Barbosa, E.T., Rodrigues, A.R., Brandão, R.S., Ulhoa, C.J., Junior, M.L., 2013. Cell wall-degrading enzymes and parasitism of sclerotia are key factors on field biocontrol of white mould by Trichoderma spp. Biol. Control. 67, 308–316.

Gouba, N., Raoult, D., Drancort, M., 2013. Plant and Fungal diversity em gut microbiota as revealed by molecular and culture investigation. PLoS One. 8, 3.

Jones, E.E., Stewart, A., Whipps, J.M., 2011. Water potential affects Coniothyrium minitans growth, germination and parasitism of Sclerotinia sclerotiorum. Fungal Biol. 115, 871–88.

Khot, L.R., Sankaran, S., Maja, E.R., Schuster, E.W., 2012. Applications of nanomaterials in agricultural production and crop protection: A review. Crop Protect. 35, 64-70.

Lacombe, S., Bradley, R.L., Hamel, C., Beaulieu, C., 2009. Do tree-based intercropping systems increase the diversity and stability of soil microbial communities? Agric., Eco. & Environ. 131, 25-31.

Lahlali, R., Hijri, M., 2010. Screening, identification and evaluation of potential biocontrol fungal endophytes against Rhizoctonia solani AG3 on potato plants. FEMS Microbiol. Lett. 311, 152–159.

Luang, S.J., Houbrake, J., Doorn, T., Hong, S.B., Borma, A.M., Hywel-Jone, N.L., Samson, R.A., 2011. Purpureocillium, a new genus for the medically important Paecilomyces lilacinus. FEMS Microbiol. Lett. 321, 141–149.

Manohar, C.S., Raghukumar, C., 2013. Fungal diversity from various marine habitats deduced through culture-independent studies. FEMS Microbiol. Lett. 341, 69–78.

Mariano, R.L.R., 1993. Métodos de seleção in vitro para o controle microbiológico de patógenos de plantas. Revisão Anual de Patologia de Plantas. 1, 369-409.

Meireles, B.B., Oliveira, S.M.A., Coelho, R.S.B., Beserra, J.C.A., 2009. Identification and genetic variability of Colletotrichum isolates causing antrhacnose in inflorescence of ornamental tropical plants. Ciênc. Rural. 39, 6.

Mendes, I.C., Hungria, M., Reis, J., Fernandes, M.F., Chaer, G.M., Mercante, F.M., Zilli, J.E., 2009. Bioindicadores para avaliação da qualidade dos solos tropicais: utopia ou realidade? Planaltina, DF: Embrapa Cerrados, 31.

Moreira, F.M.S., Siqueira, J.O., 2006. Microbiologia e Bioquímica do solo, 2 Editora UFLA, 729.

Pérez-Silvera, A.E., Valdebenito-Sanhueza, R.M., Santos, P. Felippeto, J., 2010. Controle do mofo cinzento com Clonostachys rosea na produção de mudas de fúcsia, Trop. Pl. Pathol. 35, 3.

Raeder, U., Broda, P., 1985. Rapid preparation of DNA from filamentous fungi. Lett. Appl. Microbiol. 1, 17–20.

Santos, C.C., Oliveira, F.A., Santos, M.S., Talamini, V., Ferreira, J.M.S., Santos, F.J., 2012. Influência de Trichoderma spp. sobre o crescimento micelial de Thielaviopsis paradoxa. Scientia Plena. 8(4), 1–5.

Silva, J.A.R., Araújo, A.A., Lourenço, J., SANTOS, N., Garcia, A.R., Nahúm, B.S., 2012. Conforto térmico de búfalas em sistema silvipastoril na Amazônia Oriental. Pesq. Agropec. Bras. 46, 1364–1371.

Silva, A.P., Babujia, L.C., Matsumoto, L.S., Guimarães, M.F., Hungria, M., 2013. Microbial diversity under different soil tillage and crop rotation systems in an oxisol of southern Brazil. Open Agricola. 7, 40–47.

Soleiro, C.A., Pena, G.A., Cavaglieri, L.R., Coelho, I., Kelles, L.M., Dalcero, A.M., Rosa, C.A.R., 2013. Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers. Lett. Appl. Microbiol. 57, 484–491.

Souza, R.C., Cantão, M.E., Vasconcelos, A.T.R., Nogueira, M.A., Hungria, M., 2013. Soil metagenomics reveals differences under conventional and no-tillage with crop rotation or succession. Appl. Soil Ecol. 72, 49–61.

Stumpf, R., Santos, J., Gomes, L.B., Silva, C.N., Tessmann, D.J., Ferreira, F.D., Machinski, M.J., Del, E. M., 2013. Fusarium species and fumonisins associated with maize kernels produced in Rio Grande do Sul State for the 2008/09 and 2009/10 growing seasons. Braz. J. Microbiol. 44, 89–95.

Tamura, K., Dudley, J., Nei, M., Kumar, S., 2007. MEGA 4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molec. Biol. and Evol. 24, 1596–1599.

Teixeira, H., Júnior, T.J.P., Vieira, R.F., Silva, M.B., Ferro, C.G.F., Lehner, M.S., 2012. Trichoderma spp. decrease Fusarium root rot in common bean. Summa Phytopathol. 38, 4.

Tótola, M.R., Chaer, G.M., 2002. Microorganismos e processos microbiológicos como indicadores da qualidade dos solos. In: Avarez VH, Schaefer CEGR, Barros NF, Mello J & Costa LM (Ed.) Tópicos em Ciência do Solo, v.2 Viçosa: Sociedade Brasileira de Ciência do Solo. 195–276.

Tonin, R.F.B., Reis, E.M., Delli, A.L.D., 2013. Etiologia e quantificação dos agentes causais de manchas foliares na cultura do trigo nas safras 2008 a 2011. Summa Phytopathol. 39(2), 102–109.

Trencenti, M.C., Hass, G., 2008. Integração lavoura-pecuária-silvicultura: boletim técnico / Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Desenvolvimento Agropecuário e Cooperativismo. Brasília MAPA/SDC, 54.

Trichodel® (ECCB, 2013) available in: http://www.eccb.com.br.

Trichodermil® (Itaforte, 2013) available in: http://www.itafortebioprodutos.com.br.

Vallejo, V.E., Arbeli, Z., Ter_an,W., Lorenz, N., Dick, R.P., Roldan, F., 2012. Effect of land management and Prosopis juliflora (Sw.) DC trees on soil microbial community and enzymatic activities in intensive silvopastoral systems of Colombia. Agric, Eco. & Environ. 150, 139-148.

Veiga, T., Solis-Escalante, D., Romagnoli, G., Pierick, A.T., Hanemaaijer, M., Deshmukh. D., Wahl, A., Pronk, J.T. Daran, J.M., 2013. Resolving Phenylalanine Metabolism Sheds light on natural synthesis of Penicillin G in Penicillium chrysogenum. Eukaryot. Cell. 12, 151.

Vinale, F., Nigro, M., Sivasithamparam, K., Flematti, G., Ghisalberti, E.L., Ruocco, M., Varlese, R., Marra, R., Eid, A., Woo, S.L., Loriti, M., 2013. Harzianic acid: a novel siderophore from Trichoderma harzianum. FEMS Microbiol. Lett. 347, 123–129.

Wang, Y., Yang, P., Cui, F., Kang, L., 2013. Altered Immunity in Crowded Locust Reduced Fungal (Metarhizium anisopliae) Pathogenesis. PLoS Pathogens. 9, 1–12.

Xiao, Y., Li, H.L., Li, C., Wang, J.X., Li, J., Wang, M.H.Y., 2013. Antifungal Screening of Endophytic Fungi from Ginko biloba for Discovery of Potent anti-phytopathogenic fungicides. FEMS Microbiol. Lett. 339, 130–136.

White, T.J., Bruns, T., Lee, S., Taylor, J.W., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, eds. Innis, M.A., Gelfand, D.H., Sninsky, J.J., White, T.J., Academic Press, Inc., New York, 315-322.

Zilli, J.D., Pereira, G.M.D., Júnior, I.F., Silva, K., Hungria. M., Rouws, J.R.C., 2013 Dinâmica de rizóbios em solo do cerrado de Roraima durante o período de estiagem. Acta Amazonica. 43, 2, 153–160.

Zhang, T., Zhang, Y.Q., Liu, H.Y., Wei, Y.Z., Li, H.L., Su, J., Zhao, L.Z., Yu, L.Y., 2013. Institute of Medicin Diversity and cold adaptation of culturable endophytic fungi from bryophytes in the Fildes Region, King George Island, maritime Antarctica. FEMS Microbiol. Lett. 341, 52–61.

Zeng, W., Kirk, W., Hao, J., 2012. Field management of Sclerotinia stem rot of soybean using biological control agents. Bio. Control. 60, 141–147.

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Publicado

2020-05-29

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Berber, G. C. M., Bonaldo, S. M., Carmo, K. B., Garcia, M., Farias Neto, A., & Ferreira, A. (2020). Integrated production systems revealing antagonistic fungi biodiversity in the tropical region. Scientific Electronic Archives, 13(6), 46–56. https://doi.org/10.36560/13620201150

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Ciências Agrárias

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