Apis mellifera africanized queens tolerant to the neonicotinoid thiamethoxam

Autores

  • W. C. S. Pizzaia Universidade Estadual de Maringá
  • N. C. Pereira Universidade Estadual de Maringá – Department of Biotechnology, Genetics and Cell Biology
  • T. O. Diniz Universidade Estadual de Maringá – Department of Biotechnology, Genetics and Cell Biology
  • V. A. A. Toledo Universidade Estadual de Maringá – Department of Animal Science
  • M. C. C. Ruvolo-Takasusuki Universidade Estadual de Maringá – Department of Biotechnology, Genetics and Cell Biology

DOI:

https://doi.org/10.36560/14220211292

Palavras-chave:

Genetic toxicology, apiculture, queen selection, mortality, thiamethoxam

Resumo

Interaction between bees and plants ensured success in cross-pollination crops, increasing the vigor of the species, as the production of fruits and seeds. Bees are the main pollinators of angiosperms and several crops. Among the factors related to the disappearance of bees in agricultural areas is the excessive or improper use of insecticides. However, agriculture is extremely dependent on pesticides for pest control and the neonicotinoid thiamethoxam is one of the most used insecticides. The use of these chemicals affects the pollinating bee Apis mellifera. Thus, the aim of the study was to select Africanized A. mellifera queens tolerant to thiamethoxam, producing four generations of bees kept in chronic contamination and perform tests to validate tolerance. Colonies of A. mellifera were subjected to chronic contamination by contact with thiamethoxam at a sublethal concentration of 1.65 mg a.i./L. From the surviving colonies, four generations of new queens were produced, kept in chronic contamination with thiamethoxam. Chronic contamination caused changes in the workers' behavior for 25 days. Validation of selection and tolerance was performed in vitro bioassays to verify mortality and critical electrolyte concentration (CEC). In vitro bioassays showed that there was low mortality after contamination for 24 hours by contact with thiamethoxam at 82.5 mg a.i / L. The analyzes of CEC indicate that there was an increase in gene expression in brain cells, probably as an attempt to detoxify the workers' organism by contamination with thiamethoxam. New tests need to be carried out with colonies tolerant to the neonicotinoid thiamethoxam, but these initial results indicate that these queens have potential to tolerate this neonicotinoid.

Referências

ARATHI, H.S., BURNS, I., SPIVAK, M. Ethology of hygienic behaviour in the honey bee, Apis mellifera L. (Hymenoptera: Apidae): behavioural repertoire of hygienic bees. Journal of Ethology, Vol. 106, p. 365-379, 2000.

BIENEFELD, K. Breeding Success or Genetic Diversity in Honey Bees? Bee World, Vol. 93, p. 40-44, 2016.

BIENEFELD, K., ZAUTKE, F., GUPTA, P. A novel method for undisturbed long-term observation of honey bee (Apis mellifera) behavior – illustrated by hygienic behavior towards varroa infestation. Journal of Apicultural Research, Vol. 54, p. 541-547, 2016.

BIANCHINI, L., BEDENDO, I.P. Efeito antibiótico da própolis sobre bactérias fitopatogênicas. Scientia Agricola, Vol. 55, p. 149-152, 1998.

BLAQUIÈRE, T., SMAGGHE, G., VAN-GESTEL, C. A., MOMMAERTS, V. Neonicotinoids in bees: a review on concentrations, side-effects and risk assessment. Ecotoxicology, Vol. 21, p. 973-992, 2012.

BOURGEOIS, A.L., RINDERER, T., GUZMAN, L.I., HOLLOWAY, B. Molecular genetic analysis of Varroa destructor mites in brood, fallen injured mites, and worker bee longevity in honey bees. Journal of Apicultural Research, Vol. 54, p. 328-334, 2016.

CAMPOS, J.C.P. Melhoramento genético aplicado à produção animal. Belo Horizonte - MG: FEPMVZ, 2008. 617p.

CARVALHO, S.M., CARVALHO, G.A., CARVALHO, C.F., BUENO-FILHO, J.S.S., BAPTISTA, A.P.M. Toxicidade de acaricidas/inseticidas empregados na citricultura para a abelha africanizada Apis mellifera L., 1758 (hymenoptera: apidae). Arquivos do Instituto Biológico, Vol. 76, p. 597-606, 2009.

CHERUIYOT, S.K., LATTORFF, H.M.G., KAHUTHIA-GATHU, R., MBUGI, J.P., MULI, E. Varroa-specific hygienic behavior of Apis mellifera scutellata in Kenya. Apidologie, Vol. 49, p. 439-449, 2018.

COUTO, R.H.N., COUTO, L.A. Apicultura: manejo e produtos. Jaboticabal: FUNEP, 2002. 191p.

CHRISTEN, V., SCHIRRMANN, M., JUERG, E.F., KARL, F. Global Transcriptomic Effects of Environmentally Relevant Concentrations of the Neonicotinoids Clothianidin, Imidacloprid, and Thiamethoxam in the Brain of Honey Bees (Apis mellifera). Environmental Science Technology, Vol. 52, p. 7534-7544, 2018.

FAIRBROTHER, A., PURDY, J., ANDERSON, T., FELL, R. Risks of neonicotinoid insecticides to honeybees. Environmental Toxicology and Chemistry, Vol. 33, p. 719-731, 2014.

FISCHER, J., MULLER, T., SPATZ, A.K., GREGGERS, U., GRUNEWALD, B., MENZEL, R. Neonicotinoids interfere whit specific components of navigation in honeybees. Plos One, Vol. 9, p. 1-10, 2014.

FORFERT, N., TROXLER, A., RETSCHNIG, G., GAUTHIER, L., STRAUB, L., MORITZ, R.F.A., NEUMAN, P., WILLIAMS, G.R. Neonicotinoid pesticides can reduce honeybee colony genetic diversity. Plos One, Vol. 12, p. 1-14, 2017.

GONTIJO, A.M.M.C., TICE, R. Teste do cometa para a detecção de dano no DNA e reparo em células individualizadas. In: RIBEIRO, L.R., SALVADORI, D.M.F., MARQUES, E.K. Mutagênese Ambiental. Canoas: Ulbra, 2003. p. 173-200

GUPTA, R.K., REYBROECK, W., LAGET, D., EERENS, J., LANDSHEERE, P., PAUW, M. Techniques in Beekeeping. In: GUPTA, R.; REYBROECK, W.; VAN VEEN, J.; GUPTA, A. (eds) Beekeeping for Poverty Alleviation and Livelihood Security. Springer: Dordrecht, 2014. p.557-597.

HAO, Y., LI, J. Proteomic Research on Honeybee Diseases. In: SALEKDEH, G. (eds). Agricultural Proteomics. Springer, 2016. p. 289-298.

INGRAM, D.S., GEMMILL-HERREN, B. Pollination services to agriculture: sustaining and enhancing a key ecosystem service. Food Security, Vol. 10, p. 1669-1672, 2018.

KNUTSON, R.D. Economic impacts of reduced pesticide use in the United States: measurement of costs and benefits. Agricultural and Food Policy Center, 1999. 26p

LAURINO, D., MANINO, A., PATETTA, A., PORPORATO, M. Toxicity of neonicotinoid insecticides on different honey bee genotypes. Bulletin of Insectology, Vol. 66, p. 119-126, 2013.

LECHENET, M., DESSAINT, F., PY, G., MAKOWSKI, D., MUNIER-JOLAIN, N. Reducing pesticide use while preserving crop productivity and profitability on arable farms. Nature Plants, Vol. 3, p. 1-6, 2017.

LECLERCQ, G., FRANCIS, F., GENGLER, N., BLACQUIÈRE, T. Bioassays to Quantify Hygienic Behavior in Honey Bee (Apis mellifera L.) Colonies: A Review. Journal of Apicultural Research, Vol. 57, p. 663-673, 2018.

LECLERCQ, G., PANNEBAKKER, B., GENGLER, N., NGUYEN, B. K., FRANCIS, F. Drawbacks and benefits of hygienic behavior in honey bees (Apis mellifera L.), a review. Journal of Apicultural Research, Vol. 56, p. 366-375, 2017.

LU, C., WARCHOL, K.M., CALLAHAN, R.A. Sub-lethal exposure to neonicotinoids impaired honey bees winterization before proceeding to colony collapse disorder. Bulletin of Insectology, Vol. 67, p. 125-130, 2014.

LU, C., WARCHOL, K.M., CALLAHAN, R.A. In situ replication of honey bee colony collapse disorder. Bulletin of Insectology, Vol. 65, p. 99-106, 2012.

MARTINEZ, O.A., SOARES, A.E.E. Melhoramento genético na apicultura comercial para produção da própolis. Revista Brasileira de Saúde e Produção Animal, Vol. 13, p. 982-990, 2012.

MCFARLAND, D. The complex behaviour of honeybees. In: LONGMAN, A.W. Animal behaviour: psychobiology, ethology and evolution. Oxford: University of Oxford Press,1993. p. 441-461.

MELLO, M.L.S. Cytochemistry of DNA, RNA and nuclear proteins. Brazilian Journal Genetics, Vol. 20, p. 257-264, 1997.

MICHENER, C.D. The social behavior of the bees: a comparative study. Cambridge, Massachusetts: The belknap press of harvard university press, 1974.

OLIVEIRA, A.C., JUNQUEIRA, C.N., AUGUSTO, S.C. Pesticides affect pollinator abundance and productivity of sunflower (Helianthus annuus L.), Journal of Apicultural Research, Vol. 58, p. 2-8, 2018.

PACÃFICO-DA-SILVA, I., MELO, M.M., BLANCO, B.S. Efeitos tóxicos dos praguicidas para abelhas. Revista Brasileira de Higiene e Saúde Animal, Vol. 10, p. 142-157, 2016.

PISA, L., GOULSON, D., YANG, E.C., GIBBONS, D., SÃNCHEZ-BAYO, F., MITCHELL, E., AEBI, A., SLUIJS, J., MACQUARRIE, C.J.K., GIORIO, C., LONG, E.Y., MCFIELD, M., LEXMOND, M.B., BONMATIN, J.M. An update of the Worldwide Integrated Assessment (WIA) on systemic insecticides. Part 2: impacts on organisms and ecosystems. Environmental Science and Pollution Research, Vol. 24, p.1-49, 2017.

SANDROCK, C., TANADINI, M., TANADINI, L.G., FAUSER-MISSLIN, A., POTTS, S.G. Impact of Chronic Neonicotinoid Exposure on Honeybee Colony Performance and Queen Supersedure. Plos One, Vol. 9, p. 1-13, 2014.

SRIDHARAN, G., SHANKAR, A.A. Toluidine blue: A review of its chemistry and clinical utility. Journal of Oral Maxillofacial Pathology, Vol. 16, p. 251-255, 2012.

STANLEY, J., SAH, K., SUBBANNA, A.R.N.S. How efficient is the Asian honey bee, Apis cerana in pollinating mustard, Brassica campestris var. toria? Pollination behavior, pollinator efficiency, pollinator requirements and impact of pollination. Journal of Apicultural Research, Vol. 56, p. 439-451, 2017.

STEIN, K. COULIBALY, D., STENCHLY, K., GOETZE, D., POREMBSKI, S., LINDNER, A., KONATÉ, S., LINSENMAIR, E.K. Bee pollination increases yield quantity and quality of cash crops in Burkina Faso, West Africa. Scientific Reports, Vol. 7, 17691, 2017.

TAVARES, D.A., ROAT, T.C., SILVA-ZACARIN, C.M., NOCELLI, R.C.F., MALASPINA, O. Exposure to thiamethoxam during the larval phase affects synapsin levels in the brain of the honey bee. Ecotoxicology and Environmental Safety, Vol. 169, p. 523-528, 2019.

TONG, Z.Y., HUANG, S.Q. Safe sites of pollen placement: a conflict of interest between plants and bees? Oecologia, Vol. 186, p. 163-171, 2017.

WOOD, S.C., KOZI, I.I.V., KOZIV, R.V., EPP, T., SIMKO, E. Comparative chronic toxicity of three neonicotinoids on New Zealand packaged honey bees. PLoS ONE, Vol. 13, p. 1-19, 2018.

ZAKOUR, K.M, BIENEFELD, K. Basic considerations in the development of breeding plans for honey bees, illustrated by data on the native Syrian honey bee (Apis mellifera syriaca), Journal of Apicultural Research, Vol. 53, p. 314-326, 2014.

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Publicado

2021-01-26

Como Citar

Pizzaia, W. C. S., Pereira, N. C., Diniz, T. O., Toledo, V. A. A., & Ruvolo-Takasusuki, M. C. C. (2021). Apis mellifera africanized queens tolerant to the neonicotinoid thiamethoxam. Scientific Electronic Archives, 14(2), 35–42. https://doi.org/10.36560/14220211292

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