Antifungical and immunomodulatory activities from Caesalpinia pulcherrima extracts

Autores

  • Ricardo Sérgio da Silva Universidade Federal de Pernambuco
  • Dayane Kelly Dias do Nascimento Santos Universidade Federal de Pernambuco
  • Georon Ferreira de Sousa Universidade Federal de Pernambuco
  • Marcela de Araújo Sobral Universidade Federal de Pernambuco
  • Paulo Henrique Oliveira de Miranda Universidade Federal de Pernambuco
  • Bruna Rodrigues de Sousa Universidade Federal de Pernambuco
  • Pérsio Alexandre da Silva Universidade Federal de Pernambuco
  • Norma Buarque de Gusmão Universidade Federal de Pernambuco
  • Gláucia Manoella de Souza Lima Universidade Federal de Pernambuco
  • Márcia Silva do Nascimento Universidade Federal de Pernambuco
  • Iranildo José da Cruz Filho Universidade Federal de Pernambuco
  • Túlio Diego da Silva Centro de Tecnologias Estratégicas do Nordeste
  • Reginaldo Gonçalves de Lima Neto Universidade Federal de Pernambuco
  • Cristiane Moutinho Lagos de Melo Universidade Federal de Pernambuco

DOI:

https://doi.org/10.36560/14920211454

Palavras-chave:

Anti-inflammatory profile; Antioxidant; Antimicrobial; Immunostimulation

Resumo

Caesalpinia pulcherrima is a species widely used in folk medicine for various diseases such as fever, infections and mouth ulcers. In addition, scientific studies have reported medicinal properties such as antitumor, antiviral, antibacterial and antifungal. Therefore, the objective of this work was to evaluate if organic extracts obtained from leaves of C. pulcherrima have medicinal properties. Three organic extracts were obtained (hexane, ethyl acetate and ethanol) from the leaves collected from the Soxhlet equipment. The characterization was made by GC-MS and UPLC-MS and biological properties as antioxidant (AAT, DPPH, ABTS and lipid peroxidation radicals), antimicrobial, cytotoxicity and immunostimulant (using splenocytes of mice Balb/c) were investigated. Results showed several classes of secondary metabolites, but the ethyl acetate showed more phenols and flavonoids than the other extracts. Extracts showed good results in antioxidant tests, especially the ethyl acetate, but did not show antibacterial activity. The fungal evaluation showed high antifungal properties, especially the hexanic and the ethyl acetate against Candida tropicalis, Aspergillus terreu, Candida krusei and Cryptococcus neoformans strains. Cytotoxicity evaluation showed that under 25 μg/mL are the safety doses that can be used for in vitro tests, besides that, extracts were able to induce cytokine stimulation. These results show that the ethyl acetate extract was the best formulation found in this study to be used against fungal infections, for antioxidant proposes and for promote immunostimulation.

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Publicado

2021-08-30

Como Citar

Silva, R. S. da ., Santos, D. K. D. do N. ., Sousa, G. F. de ., Sobral, M. de A., Miranda, P. H. O. de ., Sousa, B. R. de ., Silva, P. A. da ., Gusmão, N. B. de ., Lima, G. M. de S. ., Nascimento, M. S. do, Cruz Filho, I. J. da, Silva, T. D. da ., Lima Neto, R. G. de ., & Melo, C. M. L. de . (2021). Antifungical and immunomodulatory activities from Caesalpinia pulcherrima extracts. Scientific Electronic Archives, 14(9). https://doi.org/10.36560/14920211454

Edição

v. 14 n. 9 (2021): Scientific Electronic Archives

Seção

Ciências da Saúde

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