Chemical profile and antioxidant, antibacterial, and cytotoxic activities on Artemia salina from the essential oil of leaves and xylopodium of Cochlospermum regium

Roberta Heloísa de Paula Magalhães; Antonio Carlos Pereira de Menezes Filho; Matheus Vinicius Abadia Ventura; Hellen Regina Fernandes  Batista-Ventura; Carlos Frederico de Souza Castro; Cinthia Alves Porfiro

doi:10.36560/15120221506

Autores

Roberta Heloísa de Paula Magalhães Faculdades UniBras
Antonio Carlos Pereira de Menezes Filho Instituto Federal de Educação, Ciência e Tecnologia Goiano, Campus Rio Verde
Matheus Vinicius Abadia Ventura Instituto Federal de Educação, Ciência e Tecnologia Goiano, Campus Rio Verde
Hellen Regina Fernandes Batista-Ventura Instituto Federal de Educação, Ciência e Tecnologia Goiano, Campus Rio Verde
Carlos Frederico de Souza Castro Instituto Federal de Educação, Ciência e Tecnologia Goiano, Campus Rio Verde
Cinthia Alves Porfiro Faculdades UniBras

DOI:

https://doi.org/10.36560/15120221506

Resumo

Cochlospermum regium is a shrub plant species from the Cerrado domain used in traditional medicine. This study aimed to evaluate the chemical profile and antioxidant, antibacterial and cytotoxic activities on Artemia salina from the essential oil of fresh leaves and xylopodium. Fresch leaves and xylopodium of C. regium were collected in an area of Cerrado domain in Goiás State, Brazil, 2021. The essential oil was obtained by hydrodistillation, the yield was quantified and the chemical profile determined by gas chromatography with mass spectrometry (GC-MS). Physicochemical analyzes were carried out for organoleptic analysis (color and appearance), solubility, relative density (g mL^-1), refractive index, optical rotation (α_D), antioxidant activity in DPPH radical reduction (IC₅₀ µL mL^-1), antibacterial activity on Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Salmonella serovar Enteritidis and Salmonella serovar Thyphymurium by the disc method (mm), and cytotoxicity bioassay on Artemia salina (LC₅₀ µg mL^-1). The major compounds for the essential oil of fresh leaves were viridiflorol 10.21%, Copaen-4-α-ol <β>, longiborneol 9.07 and β-bisabolene 11.48%, and for the essential oil of xylopodium β-selinene 26.17%, aromadendrene 8.66 % and thujopsene 8.09%. The yield was 0.58 and 0.33%, color slightly yellow and yellow for fresh leaves and xylopodium, respectively. Positive solubility, refractive index of 1.3468 and 1.3347, optical rotation +48.8 and +21.5, relative density 0.932 and 0.936 g mL^-1, antioxidant activity IC₅₀ = 47.65 and 111.16 µL mL^-1 for fresh leaves essential oil and xylopodium, respectively. The essential oil from fresh leaves showed high antibacterial potential for all strains, as well as for cytotoxic activity on A. salina with LC₅₀ = 90.17 and 625.08 µg mL^-1, respectively.

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