Identification of female genital tract infectious agents in patients treated for cytopathological diagnosis

J. S. Borges, V. M. Giudice, R. N. Leandro, K. V. Martinez, L. P. R. Silva, E. S. Garcia, C. G. Santos, P. A. Pereira, F. C. Charles, L. C. Mezzomo, L. N. Calil, A. Mezzari

Resumo


Vaginal candidiasis, fungal infection caused by species of Candida spp. that affects women of all ages, cultures, habits, social and economic conditions. The present study had as its main objective to determine the identification of Candida spp. isolated in a vaginal sample, collected together with the collection for the cytopathological exam, in women treated in a university extension project, aiming at allowing a suitable therapeutics afterwards. The extension project began in January 2014 to June 2019 at the UFRGS. The patients who sought the Laboratory of Clinical and Toxicological Analysis (LACT) of the Faculty of Pharmacy of UFRGS first answered a questionnaire to obtain epidemiological data. Cytopathological diagnosis, Papanicolao smear, and presence of Candida spp. yeast, culture and identification and Trichomonas vaginalis, light microscopy. During the study period, 227 patients, 25.11%, aged 15 to 82 years, presented positive culture for Candida spp. Six species were identified, C. albicans (40.35%), C. glabrata (28.07%) and C. parapsilosis, 15.79%. C. krusei, C. guilliermondii and C. tropicalis totaled (15.79%). Regarding the inflammatory process, present in 49.12%, in C. krusei (75%) and C. guilliermondii (100%), and absence in C. tropicalis. The correct diagnosis and treatment of patients with vaginal candidiasis, even if not considered sexually transmitted, enables to prevent contamination through direct contact, sexual or otherwise, with other individuals and the improvement of self-esteem and quality of life. The prevalence of Candida albicans is still the main cause of vaginal candidiasis, but not neglecting the increase in the number of cases associated with non-albicans species, as well as other infectious agents such as Trichomonas vaginalis, and bacteria.

 



Palavras-chave


Candidiasis, Candida spp., Papanicolao, Diagnosis.

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Referências


ALVARES, CA., SVIDZINSKI, TIE., CONSOLARO, MEL. Candidíase vulvovaginal: fatores predisponentes do hospedeiro e virulência das leveduras. J Bras Patol Med Lab 43(5):319-27, 2007.

ALVES, MB., SILVA, IMO., SANTOS, CI., FRANÇA, YR., OLIVEIRA, SKR., MONTEIRO, SG., MONTEIRO, CA. Prevalência de Candida spp. em amostras de secreção vaginal e sua relação com fatores associados à vulvovaginite. Revista de Investigação Biomédica 7(1):58-68, 2015.

BACKES, LTH., MEZZARI, A., CALIL, LN. População feminina atendida no sus e prevalência de alterações citológicas no colo do útero. Extensio: R. Eletr. de Extensão 13(21):56-67, 2016.

BARRENETXEA, ZG. Vulvovaginitis candidiásica. Rev Iberoam Micol 19:22-4, 2002.

BITEW, A., ABEBAW, Y. Vulvovaginal candidiasis: species distribution of Candida and their antifungal susceptibility pattern. BMC Women's Health 18(1):94, 2018.

BRANDOLT, TM., KLAFKE, GB., GONÇALVES, CV., BITENCOURT, LR., MARTINEZ, AMB., MENDES, JF., MEIRELES, MCA., XAVIER, MO. Prevalence of Candida spp. in cervical-vaginal samples and the in vitro susceptibility of isolates. Brasilian Journal of Microbiology 48:145-150, 2017.

BRASIL. MINISTÉRIO DA SAÚDE. Política Nacional de Atenção Integral à Saúde da Mulher: Princípios e Diretrizes. [Internet]. 2004 [cited 2019 Dec 24]. Available from:

http://bvsms.saude.gov.br/bvs/publicacoes/politica_nac_atencao_mulher.pdf.

BRASIL. SECRETARIA DE ATENÇÃO À SAÚDE: Departamento de Atenção Básica. Controle dos cânceres do colo do útero e da mama. Cadernos de Atenção Básica, n. 13 [Internet]. 2013 [cited 2019 Nov 15]. Available from:

http://bvsms.saude.gov.br/bvs/publicacoes/controle_canceres_colo_utero_2013.pdf.

FERRAZA, MH., MALUF, M., CONSOLARO, M.E., SHINOBU, C., SVIDZINSKI, TIE., BATISTA, MR., Characterization of yeasts isolated from the vagina and their association with vulvovaginal candidíasis in two cities of the South of Brazil. Rev Bras Ginecol Obstet 27(2):58-63, 2005.

FEUERSCHUETTE, OHM., SILVEIRA, SK., FEUERSCHUETTE, I., CORRÊA, T., GRANDO, L., TREPANI, A. Candidíase vaginal recorrente: manejo clínico. FEMINA 38(2):31-6, 2010.

GONÇALVES, B., AZEVEDO, NM., HENRIQUES, M., SILVA, S. Hormones modulate Candida vaginal isolates biofilm formation and decrease thei susceptibility to azoles ans hydrogen peroxide. Medical Mycology 2019, myz070, https://doi.org/10.1093/mmy/myz070.

PEREIRA, MS. Influência da microbiota vaginal na incidência de lesões intraepiteliais cervicais HPV – induzidas. Dissertação do curso de pós graduação em Ciências Biológicas, Juiz de Fora, 2018.

RIBEIRO, MA., DIETZE, R., PAULA, CR., DA MATTA, DA., COLOMBO, AL. Susceptibility profile of vaginal yeast isolates from Brazil. Mycopathologia 151:5-10 2001.

ROSA, MI., RUMEL, D. Fatores associados à candidíase vulvovaginal: estudo exploratório. Rev Bras Ginecol Obstet 26(1):65-70, 2004.

SIMÕES, JA. The diagnosis of vaginal candidiasis. Rev Bras Ginecol Obstet 27(5):233-234, 2005.

TABILE, PM., LUCENA, H., CHAVES, J., FISCHBORN, J., JUCÁ, RB. Características clínicas, prevalência e diagnóstico de vulvovaginites em ambulatório do interior do Rio Grande do Sul: dados e importância do diagnóstico. Journal of Health and Biological Sciences 4(3):160-65, 2016.

TOZZO, AB., GRAZZIOTIN, NA. Candidíase vulvovaginal. Perspectiva 36(133):53-62, 2012.

de Extensão Universitária. Rev Bras Ciências da Saúde 21(2):151-56, 2017.

NARANG, T., BHATTACHARJEE, R., SINGH, S., JHA, K., KAVITA, MR., DOGRA, S. Quality of life and psychological morbidity in patients with superficial cutaneous dermatophytosis. Mycoses 62(8):680-685, 2019.

ROCHA, LF., BITTENCOURT, FM., HERNANDES, KM., GONÇALVES, SMB., RODRIGUES, CMG., BERGAMO, VZ,, CALIL, LN., MEZZARI, A. Epidemiological profile of cutaneous superficial mycoses in South, Brazil. Sci Elec Arch 11(2):133-37, 2018.

SIDRIM, JJC,, ROCHA, MFG. Micologia médica à luz de autores contemporâneos. Rio de Janeiro: Guanabara Koogan; 2010.

TAKAHASHI, JPF., BECEGATO, EZ., ROCHA, N., KIMURA, LM., FERNANDES, KR., GUERRA, JM. A biologia molecular como ferramenta para identificação fúngica em amostras de tecido. Bol Inst Adolfo Lutz 26(U):1-2, 2016.

VIJENDRAN, P., VERMA, R., HAZRA, N., VASUDEVAN, B., DEBDEEP, M., RUBY, V., SHEKAR, N. A comparative study of the various patterns of oro-cutaneous fungi and their sensitivity to anti fungal between HIV patients and normal healthy individuals. Med J Armed Forces India 75(1):50-57, 2019.




DOI: http://dx.doi.org/10.36560/13920201079

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