Geographical distribution, diversity and pathogenicity of Colletotrichum associ-ated with soybean anthracnose in Brazil

S. R. G. Moraes, S. M. Bonaldo, A. R. R. Gaspareto

Resumo


Eighty-five Colletotrichum isolates were obtained from soybean croplands in 34 municipalities of Mato Grosso state. Our objectives were characterize the isolates through mycelial growth, cultural and morphological analyses, as well as assessing pathogenicity. To evaluate cultural characteristics, the mycelial growth was daily measured, and the color of colonies was characterized after ten days incubation. The morphology, length, and width of 50 conidia per isolate were assessed. The pathogenicity of twenty-one isolates was evaluated on seed germination rate, incidence, and detection of extracellular enzymes. The cultural and morphology characteristics of Colletotrichum isolates were greatly variable. All isolates studied were able to reduce seed germination, produce sign and proteinase activity. These results suggest there are more than one Colletotrichum species associated with soybean anthracnose in Mato Grosso State.


Palavras-chave


morphological and cultural traits; Glycine max

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Referências


Alfenas, A.C., Mafia, R.G. Métodos em Fitopatologia. Viçosa. Ed. UFV, 2007. 382p.

Almeida, L.C.C., Coelho, R.S.B. Caracterização da agressividade de isolados de Colletotrichum de maracujá amarelo com marcadores bioquímico, fisiológico e molecular. Fitopatologia Brasileira, v.32, n.4, p.318-328, 2007. https://doi.org/10.1590/S0100-41582007000400006

Andrade, E.M., Uesugi, C.H., Ueno, B., Ferreira, M.A.S.V. Caracterização morfocultural e molecular

de isolados de Colletrotichum gloeosporioides patogênicos ao mamoeiro. Fitopatologia Brasileira, v. 32, n.1, p.21-31, 2007. http://www.scielo.br/pdf/fb/v32n1/03.pdf

Assis, T.C., Menezes, M., Andrade, D.E.G.T., Coe-lho, R.S.B. Differentiation of Colletotrichum gloeo-sporioides isolates by using total proteins and es-terase electrophoretic patterns and extracellular enzymes production. Summa Phytopatologica, v.36, n.2, 2010. https://doi.org/10.1590/S0100-54052010000200006

Barbieri, M.C.G, Ciampi-Guillardi, M., Moraes, S.R.G., Bonaldo, S.M., Rogerio, F., Linhares, R.R., Massola Junior, N.S. First report of Colletotrichum cliviae causing anthracnose on soybean in Brazil. Plant Disease, v.101, n.9, p.1677, 2017. https://doi.org/10.1094/PDIS-07-16-0963-PDN

Begum, M.M., Sariah, M., Puteh, A.B., Zainal-Abidin, M.A. Pathogenicity of Colletotrichum trunca-tum and its influence on soybean seed quality. International Journal of Agriculture and Biology, v.10, n.4, p.393-398, 2008. ISSN Print: 1560–8530; ISSN Online: 1814–9596 07–443/MFA/2008/10–4–393–398 http://www.fspublishers.org

Canteri, M.G., Althaus, R.A., Virgens Filho, J.S., Giglioti, E.A., Godoy, C.V. SASM-Agri: Sistema para analise e separação de médias em experimentos agrícolas pelos métodos Scott Knott, Tukey e Duncan. Revista Brasileira de Agrocom-putação, v.1, p.18-24, 2001.

Castellani, A. Viability of some pathogenic fungi in distilled water. The Journal of tropical medicine and hygiene, v.42, p.225–226, 1939.

Chen, L.S., Chu, C., Liu, C.D., Chen, R.S., Tsay, J.G. PCR-based detection and differentiation of anthracnose pathogens, Colletotrichum gloeospori-oides and C. truncatum, from vegetable soybean in Taiwan. Journal of Phytopathology, v.154, p.654-662, 2006. https://doi.org/10.1111/j.1439-0434.2006.01163.x

CONAB - Companhia Nacional de Abastecimento, 2019.https://www.conab.gov.br/info-agro/safras/graos. 20 Fev. 2020.

Damm, U., O’Connell, R.J., Groenewald, J.Z., Crous, P.W. The Colletotrichum destructivum spe-cies complex - hemibiotrophic pathogens of forage and field crops. Studies in Mycology, v.79, p.49-84, 2014.

https://doi.org/10.1016/j.simyco.2014.09.003

Damm, U., Sato, T., Alizadeh, A., Groenewald, J.Z., Crous, P.W. The Colletotrichum dracaenophilum, C. magnum and C. orchidearum. Studies in Mycology, v. 92, p.1-46, 2019.

https://doi.org/10.1016/j.simyco.2018.04.001

Dias, M.D., Fonseca, M.E.N., Dias-Neto, J.J., Santos, M.D.M., Pandolfo, G.M., Boiteux, L.S., Cafe-Filho, A.C. Biology, pathogenicity, and haplotype analyses of Colletotrichum cliviae: a novel soybean anthracnose agent in warm tropical areas. Tropical Plant Pathology, v. 43, p.439-451, 2018. http://dx.doi.org/10.1007/s40858-018-0249-6

Dias, M.D., Pinheiro, V.F., Cafe-Filho, A.C. Impact of anthracnose on the yield of soybean subjected to chemical control in the north region of Brazil. Summa Phytopathologica, v.42, n.1, p.18-23, 2016. https://doi.org/10.1590/0100-5405/2114

Galli, J.A., Panizzi, R.C., Fessel, S.A., Simone, F., Ito, M.F. Efeito de Colletotrichum dematium var. truncata e Cercospora kicuchii na germinação de sementes de soja. Revista Brasileira de Sementes, v.27, n.2, p.182-187, 2005. https://doi.org/10.1590/S0101-31222005000200026

Hartman, G.L., Rupe, J.C., Sikora, E.J., Domier, L.L., Davis, J.A., Steffey, K.L. Compedium of soy-bean diseases and pets, 5th ed APS Press, St. Paul, Minnesota. 2015.

Jagtap, G.O., Sontakke, P.L. Taxonomy and morphology of Colletotrichum truncatum isolates pathogenic to soybean. African Journal of Agricultural Research, v.4, p.1483-1487, 2009.

Mahmodi, J.B., Kadir, M.Y., Wong, M.Y., Nasehi, A., Puteh, A., Soleimani, N. First Report of Anthrac-nose Caused by Colletotrichum gloeosporioides on Soybean (Glycine max) in Malaysia. Plant Disease, v.97, n.6, 2013. https://doi.org/10.1094/PDIS-10-12-0944-PDN

Majonjo, D.M., Kapooria, R.G. Ocorrence and vari-ability of Colletotrichum truncatum on soybean in Zambia. EPPO Bulletin, v.33, p.339, 2003. https://doi.org/10.1046/j.1365-2338.2003.00657.x

Munsell® Soil Color Charts, Munsell Color Compa-ny, Inc. Baltimore, Maryland 21218, USA. 1971.

Oliveira, J.A. Efeito do tratamento fungicida em sementes no controle de tombamento de plântulas de pepino (Cucumis sativas L.) e pimentão (Capsicum annanum L.). Lavras: Escola de Agricultura de Lavras, 1991. 111p. Dissertação Mestrado.

Pereira, V.W. Caracterização e identificação molecular de espécies de Colletotrichum associadas à antracnose da goiaba no Estado de São Paulo. Piracicaba:Escola Superior de Agricultura “Luiz de Queiroz”/Universidade de Sao Paulo, 2009. 79p. Dissertação Mestrado.

Ramos, A.M., Gally, M., Garcia, M.C., Levin, L. Pectinolytic enzyme production by Colletotrichum truncatum, causal agent of soybean anthracnose. Revista Iberoamericana de Micología, v. 27, p.186-180, 2010.

https://doi.org/10.1016/j.riam.2010.06.002

Ramos, A.M., Franco, T.L., Cinto, I., Carmona, M., Gally, M. Molecular characterization of Colleto-trichum species causing soybean anthracnose in Argentina. Mycotaxon, v.123, p.457-465, 2013. https://doi.org/10.5248/123.457

Redman, R.S., Rodriguez, R.J. Characterization and isolation of an extracellular serine protease from the tomato pathogen Colletotrichum coccodes, and it’s role in pathogenicity. Mycolical Research, v.106, n.12, p.1427-1434, 2002.

https://doi.org/10.1017/S0953756202006883

Riccioni, L., Conca, G., Hartman, G.L. First report of Colletotrichum coccodes on soybean in the United States. Plant Disease, v.82, n.8, p.959, 1998. https://doi.org/10.1094/PDIS.1998.82.8.959C

Rogerio, F., Ciampi-Guillardi, M., Barbieri, M.C.G., Bragança, C.A.D., Seixas, C.D.S., Almeida, A.M.R., Massola Jr., N.S. Phylogeny and variability of Colle-totrichum truncatum associated with soybean an-thracnose in Brazil. Journal of Applied Microbiology, v.122, n.2, p.402-415, 2016.

https://doi.org/10.1111/jam.13346

Salisbury, F.B., Ross, C.W Plant physiology. 4.ed. Belmont: Wadsworth, 1991. 682p.

Sutton, B.C. The Coelomycetes. Commonwealth Mycological Institute, Kew. 1980. 696p.

Torres-Calzada, C., Tapia-Tussell, R., Higuera-Ciapara, I., Huchin-Poot, E., Martin-Mex, R., Nex-ticapan-Garcez, A., Perez-Brito, D. Characterization of Colletotrichum truncatum from papaya, pepper and physic nut based on phylogeny, morphology and pathogenicity. Plant Pathology, v.64, p.821-830, 2017. https://doi.org/10.1111/ppa.12800

Tozze Jr, H.J., Firmino, A.C., Fischer, I.H., Furtado, E.L., Massola Jr, N.S. Caracterização da agressividade e atividade enzimática de isolados de Colletotrichum spp. associados à antracnose do abacate. Summa Phytopathologica, v.42, n.3, p.264-267, 2016. https://doi.org/10.1590/0100-5405/2107

USDA-United States Department of Agriculture, Foreign Agricultural Service Circular Series 2020 World Agricultural Production. Circular Series WAP 3-20 March 2020.

https://apps.fas.usda.gov/psdonline/circulars/production.pdf 20 Fev. 2020

Velho, A.C., Alaniz, S., Casanova, L., Mondino, P., Stadnik, M.J. New insights into the characterization of Colletotrichum species associated with apple diseases in southern Brazil and Uruguay. Fungal Biology, v.119, n.4, p.229-244, 2015.

https://doi.org/10.1016/j.funbio.2014.12.009

Vinnere, O. Approaches to Species Delineation in

Anamorphic (mitosporic) Fungi: A Study on Two Extreme Cases. Uppsala: Acta Universitates Upsa-lienses, 2004. 72p. Doctor of Philosophy. http://uu.divaportal.org/smash/get/diva2:163839/FULLTEXT01.pdf

Yang, H-C, Haudenshield, J.S., Hartman, G.L. First report of Colletotrichum chlorophyll causing soy-bean anthracnose. Plant Disease, v.96, n.11, p.1699, 2012. https://doi.org/10.1094/PDIS-06-12-0531-PDN

Yang, H-C, Haudenshield, J.S., Hartman, G.L. Colletotrichum incanum sp. nov., a curved-conidial species causing soybean anthracnose in USA. My-cologia, v.106, p.32-42, 2014.

https://doi.org/10.3852/13-013

Wahid, O.A.A. Occurrence of Colletotrichum anthracnose disease of guava fruit in Egypt. International Journal of Pest Management, v. 47, p.147-152, 2001.

https://doi.org/10.1080/09670870151130679




DOI: http://dx.doi.org/10.36560/13720201131

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