Effect of the Isolated Bacteria in the Biocontrol in vitro of Aspergillus sp.

Autores

  • S. VALIATI
  • E. FERRARI
  • H.F. SHIOMI

DOI:

https://doi.org/10.36560/1120125

Palavras-chave:

biological control, post-harvest, mycotoxins, peanut

Resumo

ABSTRACT: It was found in the laboratory the effect of seven bacterial isolates on mycelial growth of Aspergillus sp. in vitro, by the method of "paired cultures". The experimental design was a completely randomized design with eight treatments: T1 - control (saline 0.85%), T2 - BB1 (B. alcalophilus), T3 - BS3 (P. luminescens), T4 - BB4 (B. cereus GC. subgroup B), T5 - BB5 (S. maltophilia), T6 - BB6 (Y. bercovieri), T7 - BS5 (B. cereus GC. subgroup B) and T8 - BS6 (B. cereus GC. subgroup B) and three repetitions each. Was evaluated the diameter of the pathogen mycelial (cm) in the isolates treatments when control has reached the ends of the Petri dish. The data collected were subjected to analysis of variance by F test, using the statistical software ESTAT and the differences between means were compared by Tukey test at 5% probability. It was observed that the bacterial isolates BB1, BB4, BB5, BS5 and BS6 had no effect on pathogen control, matching the witness. Treatment with isolated BS3 showed 7.8 cm in diameter, was considered statistically equal to ineffective treatments and the best treatment. In dealing with isolated BS6 the mycelium of the pathogen had the lowest value, 6.03 cm, which was statistically superior to the others.

Keywords: biological control, post-harvest, mycotoxins, peanut.

Referências

BERG, G., ROSKOT, N., SMALLA, K. Genotypic and phenotypic relationships between clinical and environmental isolates of Stenotrophomonas maltophilia. Journal Clinical Microbiology 37: 3594-3600, 1999.

BETTIOL, W., MORANDI, M. A. B. Biocontrole de doenças de plantas: Uso e Perspectivas. Jaguaria: Embrapa Meio Ambiente, 341p. 2009.

BILGRAMI, K. S., SINHA, K. K., SINHA, A. K. Inhibition of aflatoxin production and growth of Aspergillus flavus by eugenol and onion and garlic extracts. Indian Journal of Medical Research 96: 171-175. 1992.

BOWEN, D. J., ENSIGN, J. C. Purification and characterization of a high-molecular-weight insecticidal protein complex produced by the entomopathogenic bacterium Photorhabdus luminescens. Applied and Environmental Microbiology 64: 3029-3035. 1998.

DHINGRA, O. D., COELHO NETTO, R. A. Micotoxinas em grãos. Revis Anual de Patologia de Plantas 6: 49-101. 1998.

FILHO, E. S. A. Comportamento de microbiota residente, Aeromonas hydrophila, Yersinia enterocolitica e Listeria monocytogenes incubadas em carne de atum (Thunnus albacares), estocada sob refrigeração (0c - 1c) em diferentes atmosferas modificadas. 142f. (Tese de Doutorado). Universidade Federal Fluminense, Niteroi, Rio de Janeiro, 2006.

GLARE, T. R., O Callaghan, M. Bacillus thuringiensis: Biology, ecology and safety. Chichester. John Wiley & Sons. 2000.

JANISIEWICZ, W. J., Korsten, L. Biological control of postharvest disease of fruits. Annual Review of Phytopathology 40: 411-441. 2002.

LUZ, W. C. Combinação dos tratamentos biológico e quico de sementes de milho. Fitopatologia Brasileira 28: 37-40. 2003.

MARTEZ-BLANCH, J. F., SCHEZ, G., AZNAR, R. Development of a real-time pcr assay for detection and quantification of enterotoxigenic members of Bacillus cereus group in food samples. International Journal of Food Microbiology 135: 1521. 2009.

MELO, R. A. G., MARIANO, R. L. R., MICHEREFF, S. J., MENEZES, M., COELHO, R. S. B. Controle biológico da podridão-mole do pimentão (Capsicum annum) causado por Erwinia carotovora subsp. carotovora. Summa Phytopathologica 21: 206-212. 1995.

MISHRA, D., DUBEY, N. K. Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities. Applied and Environmental Microbiology 60: 1101-1105. 1994.

REIS, G. M. Variabilidade genica de cepas de Aspergillus flavus isoladas de amendoim. 113f. (Dissertação de Mestrado). Universidade de S Paulo, S Paulo, 2009.

SANHUEZA, R. M. V. Leveduras para o controle de fitopatógenos. In: 6 Simpósio de Controle Biológico, Rio de Janeiro. Anais... Rio de Janeiro: Fiocruz, p.340-343, 2000.

SANTOS, A., FERREIRA, M. V. R., SOARES, A. E., SILVA, M. A., ALMEIDA, J. J., SHIOMI, H. F. Seleção de bactérias com ação antagônica a Sclerotinia sclerotiorum a partir de biofertilizantes. Jaguaria. Summa Phytopathologica. Jaguaria: Grupo paulista de fitopatologia, 2012. v.38, Supl. cd-rom. 2012.

VANZOLINI, S., TORRES, R. M., PANIZZI, R. C. Efeito do tamanho, da densidade e do tratamento fungicida sobre a qualidade de sementes de amendoim. Revista Ceres 47: 603-612. 2000.

YADAV, E., PATHAK, D. V., SHARMA, S. K., KUMAR, M., SHARMA, P. K. Isolation and characterization of mutants of Pseudomonas maltophilia pm-4 altered in chitinlytic activity and antagonistic activity against root rot pathogens of clusterbean (Cyampsis tetragonoloba). Indian Journal of Microbiology 47: 64-71

Downloads

Publicado

2012-11-20

Como Citar

VALIATI, S., FERRARI, E., & SHIOMI, H. (2012). Effect of the Isolated Bacteria in the Biocontrol in vitro of Aspergillus sp. Scientific Electronic Archives, 1(1), 11–15. https://doi.org/10.36560/1120125

Edição

Seção

Ciências Biológicas