Evaluation of the antimutagenic and cytotoxic activity of Morinda citrifolia Linn. (Noni)

B. H. B. Martins, W. M. Tominaga, L. Castoldi, E. A. Camargo, D. L. Pereira, E. M. J. Lisboa, L. R. Albiero, J. B. Ferreira, N. Melchiors, M. M. Sugui


Recently, Morinda citrifolia Linn., popularly known in Brazil as noni, is used in folk medicine for its therapeutic properties to prevent and cure various diseases, among them cancer. The fruit has in its composition compounds with biological activities and, consequently, have chemopreventive effects. In this context, the objective of the study was to evaluate the antimutagenic/mutagenic potential of aqueous extract of the noni through the Micronucleus Test in vivo and to investigate the possible toxic effect of noni through the in vitro Cytotoxicity Test. The animals (6 animals/group) were treated for 15 consecutive days with aqueous extract (via gavage) and on day 15th were given intraperitoneally cyclophosphamide (CPA, 25 mg/Kg) and sacrificed 24 hours after treatment for evaluate of erythrocyte frequency micronucleated polychromatic (MNPCE). The chinese hamster ovary (CHO) cells were incubated with aqueous extract of noni at the concentrations of 50, 200, 1000, 2500 and 5000 μg/ml for 3 hours and Ehrlich ascitic tumor cells (TAE) using concentrations of 1.0; 0.5 and 0.25 mg/ml of the noni extract, incubated for 24 hours. After treatment,  the cell viability test was performed with tripan blue, in triplicate. The results showed that pretreatment with aqueous extract of noni, under the conditions tested, significantly reduced the frequency of MNPCE (p<0,001) induced by CPA, when compared to the positive control group. The group treated only with noni showed no mutagenic effect. The different concentrations of the noni aqueous extract showed no toxic effect on CHO and TAE cells when compared to the control group, that is, there is a possibility of cell proliferation when exposed to noni under the conditions tested. Thus, to ensure safety of noni use, future studies should be performed to verify the mechanism of action of the isolated compounds of the aqueous extract in the prevention of DNA damage.


Antimutagenicity, DNA, cytotoxicity and Morinda citrifolia

Texto completo:



AGÊNCIA NACIONAL DE VIGILÂNCIA SANITÁRIA (ANVISA). Esclarecimento sobre as avaliações de segurança realizadas de produtos contendo Morinda citrifolia, também conhecida como Noni. Informe Técnico nº. 25, de 29 de maio de 2007. Atualizado em 18 de junho de 2008. http://www.anvisa.gov.br/alimentos/informes/25_290507.htm

BROWN, A. C. Anticancer Activity of Morinda citrifolia (Noni) fruit: A review. Phytotherapy Research 26:1427-40, 2012.

DELMANTO, R. D. et al. Antimutagenic effect of Agaricus blazei Murrill mushroon on the genotoxicity induced by cyclophosphamide. Mutation Research-Genetic Toxicology 496:15-21, 2001.

DUSSOSSOY, E. et al. Characterization, anti-oxidative and anti-inflammatory effects of Costa Rican noni juice (Morinda citrifolia L.). Journal Ethnopharmacology 133:108-115, 2011.

FERREIRA, F. G. et al. Avaliação de mutagenicidade e antimutagenicidade de diferentes frações de Pterogyne nitens (leguminosae) utilizando ensaio de micronúcleo em Tradescantia pallida. Revista Brasileira de Farmacognosia 19:61-67, 2009.

FURUSAWA, E. Anti-cancer Activity of Noni Fruit Juice Against Tumors in Mice. Proceedings of the 2002 Hawai‘i Noni Conference, University of Hawaii at Manoa, College of Tropical Agriculture and Human Resources, 2003.

FURUSAWA, E. et al. Antitumour potential of a polysaccharide-rich substance from the fruit juice of Morinda citrifolia (noni) on sarcoma 180 ascites tumour in mice. Phytotherapy Research 1:1158-1164, 2003.

JENSEN, C. J. et al. Preventative and treatment effects of Morinda citrifolia as a colon cancer cell growth inhibitor. United States Patent Application 285-334, 2003.

JIMENÉZ, M.R. et al. Genotoxicity and anti-genotoxicity of some traditional medicinal herbs. Mutation Research 585:147-155, 2005.

KUPRADINUN, P.; TEPSUWAN, A.; KUSAMRAN, W. R. Anticlastogenic effect of Asiatic Penneywort and Indian Mulberry using rodent erythrocyte Micronucleus Assay. The Thai Journal of Veterinary Medicine 41:87-94, 2011.

KUSAMRAN, W. R.; TEPSUWAN A.; KUPRADINUN, P. Antimutagenic and anticarcinogenic potentials of some Thai vegetables. Mutation Research 402:247-258, 1998.

LAVAUT, N. E. G.; LAVAUT, J. A. G. Morinda citrifolia Linn: potencialidades para su utilización em La salud humana. Revista Cubana de Farmácia 37:12-15, 2003.

LISHUANG, L.V. et al. Chemical components of the roots of Noni (Morinda citrifolia) and their cytotoxic effects. Fitoterapia 82:704-708, 2011.

MacGREGOR, J. T. et al. Guidelines for the conduct of micronucleus assay in mammalian bone marrow erythrocytes. Mutation Research 189:103-112, 1987.

MANOHAN, K., BANERJEE, M. R. β-carotene reduces sistes chromatid exchange induce chemical carcinogens in mouse mammary cells in organ culture. Cell Biol Int Rep 9:783-89, 1985.

MARQUES, N. F. G. et al. Delayed ossification in Wistar rats induced by Morinda citrifolia L. exposure during pregnancy. Journal of Ethnopharmacology 128:85-91, 2010.

MULLER, J. C. Toxicidade reprodutiva da Morinda citrifolia Linn. 103 f. Dissertação (Mestrado em Farmacologia)- Universidade Federal do Paraná, Setor de Ciências Biológicas, Curitiba - PR: 2007.

PALIOTO, G. F.; JABOR, S.; ROCHA, C. L. M. S. C. Avaliação do potencial antimutagênico do suco de Morinda citrifolia L. (Noni) em Aspergillus nidulans. In: Resumos do 55° Congresso Brasileiro de Genética, 2009.

PALU, A. K. et al.The effects of Morinda citrifolia L. (noni) on the immune system: its molecular mechanisms of action. Journal of Ethnopharmacology 115:502-506, 2008.

PALU, A. K., SU, C.; JENSEN, J. Noni (Morinda citrifolia L.) Fruit juice anti-breast cancer potential: a mechanism involving its aromatase enzyme inhibitory effect. Journal Medicinal Food Plantas 1: 55-57, 2009.

PEREIRA, C.A.B. Teste estatístico para comparar proporções em problemas de citogenética. In: RABELLO-GAY, M.N., RODRIGUES M.A, La R., Montelleone-Neto (Eds.) Mutagênese, teratogênese e carcinogênese: métodos e critérios de avaliação. São Paulo: FCA, p.113-21, 1991.

SAMOYLENKO, V. et al. New constituents from noni (Morinda citrifolia) fruit juice. Journal of Agricultural and Food Chemistry 54:6398-6402, 2006.

SANTANA, G.M. et al. Citotoxicidade do fruto de Morinda citrifolia L. sobre as células meristemáticas de Allium cepa L. 65ª. Reunião Anual da SBPC, UFPE – Recife, 16-17, 2013.

SERAFINI, M. R. et al. Pre-clinical toxicity of Morindacitrifolia Linn. leaf extract. African Journal of Biotechnology 10:14566-14572, 2011.

SUGUI, M.M. Mecanismos de antimutagenicidade do cogumelo Agaricus brasiliensis sobre lesões no DNA induzidas in vivo e in vitro. 111 f. Tese de Doutorado (Doutorado em Patologia)- Faculdade de Medicina – UNESP – Botucatu- São Paulo, Brasil, 2006.

TASKIN, E. L. et al. Apoptosis-inducing effects of Morinda citrifolia L. and doxorubicin on the Ehrlich ascites tumor in Balb-c mice. Cell biochemistry and Function, 27:542-546, 2009.

THANI, W. et al. Anti-proliferative and antioxidative activities of Thai noni/Yor (Morinda citrifolia Linn.) leaf extract. Southeast Asian Journal of Tropical Medicine and Public Health 41:482-489, 2010.

WANG, M.; SU, C. Efeito preventivo do câncer de Morinda citrifolia (noni). Anais da New York Academy of Sciences 952:161-168, 2001.

WANG, M. et al. Morinda citrifolia (Noni): A literature review and recent advances in Noni research. Acta Pharmacologica Sinica 23:1127-1141, 2002.

WATERS, M.D. et al. Antimutagenic profiles for some model compounds. Mutation Research 238:57-85, 1990.

WEST, B. J. et al. A Safety Review of Noni Fruit Juice. Journal of Food Science 71:100-106, 2006.

YANG, J. et al. Free-radical-scavenging activity and total phenols of noni (Morinda citrifolia L.) juice and powder in processing and storage. Food Chemistry 102:302–8, 2007.

YU, E. L. et al. Acute hepatotoxicity after ingestion of Morinda citrifolia (Noni Berry) juice in a 14-year-old boy. Journal of Pediatric Gastroenterology and Nutrition 53:469-470, 2011.

DOI: http://dx.doi.org/10.36560/1212019631


  • Não há apontamentos.

Universidade Federal de Rondonópolis
Avenida dos Estudantes, 5055 - Cidade Universitária
Rondonópolis - MT, 78735-901