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Scientific Electronic Archives
Agricultural Science
DOI: 10.36560/16620231508
Submitted: 2021-11-02
Published: 2023-05-30

Mitigation of water deficit in maize plants by Bacillus spp. isolated from endemic plants in caatinga

Universidade do Estado da Bahia
Universidade do Estado da Bahia
Universidade do Estado da Bahia

Abstract

The main of this work was to evaluate the effect of Bacillus spp. isolates from native plants of the Caatinga Biome in the induction of tolerance to water deficit in maize plants. Corn plants were cultivated in a germination chamber and after emergence, the plants were inoculated with 20 mL of bacterial suspension containing 109 CFU mL-1 of each isolate tested. The parameters evaluated were shoot and root length, shoot and root fresh weight and shoot and root dry weight. The data obtained were submitted to the mean test using Skott-Knott (P<0.05) using the Sisvar software. For each isolate, the capacity to produce exopolysaccharides (EPS) and indoleacetic acid (AIA) was evaluated. All isolates were able to produce EPS and AIA. Plants that were inoculated had a significant increase in most parameters evaluated, compared to the negative control. The data demonstrate the possibility of using the isolates to reduce the effects caused by the water deficit and, in this way, reduce the losses in dry agriculture caused by droughts.

References

  1. BARRETO, M. C. S; FIGUEIREDO, M. V. B.; BURITY, H. A.; SILVA, M. L. R. B.; LIMA-FILHO, J. L. Produção e comportamento reológico de biopolímeros produzidos por rizóbios e caracterização genética. Revista Brasileira Agrociência, v.17, n.2-4, p.221-227, 2011
  2. BERGAMASCHI, H.; DALMAGO, G.A.; BERGONCI, J.I.; BIANCHI, C.A.M.; MÜLLER, A.G.; COMIRAN, F.; HECKLER, B.M.M. Distribuição hídrica no período crítico do milho e produção de grãos. Pesquisa Agropecuária Brasileira, v.39, p.831-839, 2004
  3. CHOWDHURY, S.R.; BASAK, R.K.; SEN, R.; ADHIKARI, B. Production of extracellular polysaccharide by Bacillus megaterium RB-05 using jute as substrate. Bioresource Technology, Essex, v. 102, p. 6629-6632, 2011.
  4. COHEN, A.C.; TRAVAGLIA, C.N.; BOTTINI, R.; PICCOLI, P.N. Participation of abscísico acid and gibberellins produced by endophytic Azospirillum in the alleviation of drought effects in maize. Botany, v. 87, p. 455-462, 2009.
  5. DURÃES, F. O.; GOMES, E. E.; GAMA, M. X. D. S.; DOS SANTOS, F. G.; COELHO, A. M.; ALBUQUERQUE, P. E. P.; CARLOS, A. Fenotipagem associada à tolerância a seca em milho para uso em melhoramento, estudos genômicos e seleção assistida por marcadores. Sete Lagoas: (Circular Técnica, 39). Embrapa, p.17, 2004
  6. GORDON, S.A, WEBER, R.P. Colorimetric estimation of indol acetic acid. Plant Physiol. V. 26, p.192-195, 1951.
  7. GUIMARÃES, D.P; Costa F; Rodrigues MJ; Maugeri F. Optimization of dextran synthesis and acid hydrolis by surface response analysis. Braz. J. Chem. Eng. V.16, p.129-139, 1999.
  8. KAVAMURA, V. N.; SANTOS, S. N.; SILVA, J. L.; PARMA, M. M.; AVILA, L.A.; VISCONTI, A.; ZUCCHI, T. D.; TAKETANI, R. G.; ANDREOTE, F. D.; MELO,I. S. Screening of Brazilian Cacti Rhizobacteria for Plant Growth Promotion Under Drought. Microbiological Research, 2012.
  9. KIEFT, T.L. Desert environments: soil microbial communities in hot deserts. Encyclopedia of Environmental Microbiology. 2003. Disponível em: https://onlinelibrary.wiley.com/doi/full/10.1002/0471263397.env178. Acesso em 09 de maio de 2021.
  10. KUMAR, C.G. JOO, H.-S.; CHOI, J.-W.; KOO, Y.-M.; CHANG, C.-S. Purification and characterization of an extracellular polysaccharide from haloalkalophilic Bacillus spp. I-450.Enzyme and Microbial Technology, New York, v. 34, p. 673-681, 2004.
  11. LIU, S.; CHEN, X.; HE, H.; ZHANG, X.; XIE, B.; YU, Y.; CHEN, B.; ZHOU, B.; ZHANG, Y. Structure and ecological roles of a novel exopolysaccharide from the artic sea ice bacterium pseudolateromonas spp. Strain SM20310. Appl. Environ. Microbiol. v.1. p.224, 2013
  12. MARTINS, M.A. ESTIMATIVA DA PRODUTIVIDADE DA CULTURA DO MILHO NO SEMIÁRIDO BRASILEIRO, COM BASE NO MODELO AQUACROP E PREVISÃO CLIMÁTICA. Tese de doutorado. Instituto Nacional de Pesquisas Espaciais, 2017. Disponível em: http://mtc-m21b.sid.inpe.br/col/sid.inpe.br/mtc-m21b/2017/03.09.13.39/doc/publicacao.pdf. Acesso em: 11 de março de 2021.
  13. NOCKER, A.; FERNÁNDEZ, P.S.; MONTIJN, R.; SCHUREN, F. Effect of air drying on bacterial viability: a multiparameter viability assessment. Journal of Microbiological Methods, Amsterdam, v. 90, p. 86-95, 2012.
  14. PAULO E.M; VASCONCELOS MP; OLIVEIRA IS; AFFE HMJ; NASCIMENTO R; MELO IS; ROQUE MRA; ASSIS AS. Analternative method for screenin glactic acid bacteria for the production of exopolysaccharides with rapid confirmation. Ciênc. Tecnol. Aliment. V.32, p. 710-714, 2012.
  15. PAZOS, M.; HERNÁNDEZ, A. Evaluación de cepas nativas del género Azopirillum y su interacción con el cultivo del arroz. Cultivos Tropicales, v. 22, n. 4, p. 25-28, 2001.
  16. PEDRINHO, E.A.N.; JÚNIOR, R.F.G.; COMPANHARO, J.C.; ALVES, L.M.C.; LEMOS, G.M. Identificação e avaliação de rizobactérias isoladas de raízes de milho. Bragantia v.69 n.4, 2010.
  17. PEREIRA, G.R. Correlação entre as Secas e as Perdas na Agricultura de Sequeiro no Semiárido Nordestino. Instituto Nacional de Pesquisas Espaciais/Centro Regional do Nordeste. Rio Grande do Norte, 2018.
  18. RIBEIRO, V.P.; MARRIEL, I.E.; SOUSA, S.M.; PAULA , U.G.; MATTOS, B.B.; OLIVEIRA, C.A.; GOMES, E.A. Endophytic Bacillus strains enhance pearl millet growth and nutrient uptake under low-P. Braz. J. Microbiol v. 49, p. 40-46, 2018.
  19. SANTOS, A. F. J.; MARTINS, C. Y. S.; SANTOS, P. O.; CORRÊA, E. B.; BARBOSA, H. R.; SANDOVAL, A. P. S.; OLIVEIRA, L. M.; SOUZA, J. T.; SOARES, A. C. F. Diazotrophic bacteria associated with sisal (Agave sisalana Perrine ex Engelm): potential for plant growth promotion. Plant and Soil, 385:37-48, 2014.
  20. SCHUCH, R.; FISCHETTI, V.A. The secret life of the anthrax agent Bacillus anthracis: bacteriophage-mediated ecological adaptations. PLoS ONE, San Francisco, v. 4, n. 8, p. 1-23, 2009.
  21. SILVA et. al. Caracterização do Semiárido brasileiro: fatores naturais e humanos. In: SA, I. B.; SILVA, P. C. G. da. (Ed.). Semiárido brasileiro: pesquisa, desenvolvimento e inovação. Petrolina: Embrapa Semiárido, 2010.
  22. SPAEPEN, S.; VANDERLEYDEN, J.; REMANS, R. Indole-3-acetic acid in microbial and microorganism-plant signaling, FEMS Microbiology Reviews , V.31, P. 425-448, 2007.
  23. SUTHERLAND, I.W. Biotechnology of Microbial Exopolysaccharides. Cambridge University Press, 1990.
  24. TOLEDO, F.L.; GONZALEZ-LOPEZ, J.; CALVO, C. Production of bioemulsifier by Bacillus subtilis, Alcaligenes faecalis and Enterobacter species in liquid culture. Bioresource Technology, v. 99, p. 8470-8475, 2008.
  25. VERMA, J.P.; JAISWAL, D.K.; KRISHNA, R.; PRAKASH, S.; YADAV, J.; SINGH, V. Characterization and screening of thermophilic Bacillus strains for developing plant growth promoting consortium from hot spring of Leh and Ladakh Region of India. Front. Microbiol v. 9, p. 1293, 2018.
  26. YUAN, S.J.; SUN, M.; SHENG, G.-P.; LI, Y.; LI, W.-W.; YAO, R.-S.; YU, H.-Q. Identification of key constituents and structure of the extracellular polymeric substances excreted by Bacillus megaterium TF10 for their flocculation capacity. Environmental Science and Technology, Washington, v. 45, p. 1152-1157, 2011.
  27. ZAIED, K. A. et al. Yield and Nitrogen Assimilation of Winter Wheat Inoculated with New Recombinant Inoculants of Rhizobacteria. Pakistan Journal of Biological Sciences, v. 6, n. 4, p. 344–358, 1 fev. 2003.
  28. ZHAO, Y. Auxin Biosynthesis and Its Role in Plant Development. Annual Review of Plant Biology, v. 61, n. 1, p. 49–64, 2 jun. 2010.
  29. .

How to Cite

Pereira da Silva, E., Costa Barros, Y. ., & Feitoza de Jesus Santos, A. . (2023). Mitigation of water deficit in maize plants by Bacillus spp. isolated from endemic plants in caatinga. Scientific Electronic Archives, 16(6). https://doi.org/10.36560/16620231508