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DOI: 10.36560/15820221603
Submitted: 2022-05-30
Published: 2022-10-01

Main methods of DNA sequencing

Universidade de Cuiabá
Universidade de Cuiabá
Universidade de Cuiabá
Sequencing, DNA, Biotechnology, Genome

Abstract

Deoxyribonucleic acid (DNA) sequencing is the result of a series of biochemical procedures whose main purpose is to determine the sequence of nitrogenous bases, which are: adenine (A), guanine (G), cytosine (C) and thymine (T). DNA sequencing is extremely important because it is used in the identification, diagnosis and treatment of diseases and mainly in biotechnological production to develop new products and services. Currently, DNA sequencers are divided into 1st generation (Maxam-Gilbert and Sanger Method) which consists of radioactively labeling some free deoxynucleotides and adding them to a DNA template strand and later reading in PCR printing. In the 2nd generation (454-Roche, Illumina Genome and SOLID) it is a technique whose principle is the detection of a pyrophosphate released during the incorporation of the deoxynucleotide to the DNA template strand. In the 3rd generation (Ion Torrent) it works by releasing hydrogen ions whenever a nucleotide is attached to a DNA template strand, generating a chemical signature. In the 4th generation (Nanopore) the technique aims to identify the DNA that passes through a nanopore, where each nucleotide that passes through is registered a different electrical signal generating a molecular signature. With this it is possible to identify that over time and with the development of technology this process was being modified and improved seeking better quality and shorter sequencing time

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How to Cite

Silva, R. C. ., Lima, A., & Souza , L. C. da S. (2022). Main methods of DNA sequencing. Scientific Electronic Archives, 15(10). https://doi.org/10.36560/15820221603