Skip to main content Skip to main navigation menu Skip to site footer
Scientific Electronic Archives
Health Science
DOI: 10.36560/16120231731
Submitted: 2022-12-07
Published: 2022-12-13

Evaluation of the biological effect of Moringa oleifera Lam. in vivo

Universidade Federal de Mato Grosso – Campus Universitário de Sinop
Universidade Federal de Mato Grosso – Campus Universitário de Sinop
Universidade Federal de Mato Grosso – Campus Universitário de Sinop
Universidade Federal de Mato Grosso – Campus Universitário de Sinop
Universidade Federal de Mato Grosso – Campus Universitário de Sinop
Moringa oleifera, chemoprevention, cancer

Abstract

Moringa oleifera Lam. belongs to the Moringaceae family, popularly known in Brazil as "moringa", "lírio branco" or "quiabo-de-quina", has numerous health benefits, including nutritional and medicinal properties. The study aimed to evaluate the mutagenic/antimutagenic and cytotoxic activities of the aqueous extract of M. oleifera  against damage induced to DNA by N-ethyl-N-nitrosurea (ENU) in bone marrow cells of male Swiss mice, through of the micronucleus test and cell viability in Chinese Hamster Ovary (CHO) cell cultures, respectively. Eight animals/group were used, which received for 15 consecutive days the treatment, via gavage, with aqueous extract of M. oleifera. On the 15th day, the animals received intraperitoneal treatment with 0.9% NaCl or ENU (50 mg/Kg), being sacrificed 24 hours after treatment to evaluate the frequency of micronucleated polychromatic erythrocytes (MNPCEs). For the evaluation of cytotoxic activity in vitro of CHO cells, the trypan blue exclusion assay was used for the concentrations of 5000, 2500, 1000, 200, 100 and 50 µg/mL of the extract of M. oleifera. The results obtained show that the pretreatment with the aqueous extract of the M. oleifera, under the conditions tested, significantly reduced (p ≤ 0.01) the frequency of MNPCEs induced by ENU when compared with the positive control group, presenting antimutagenic effect. There was no mutagenic effect in the group treated only with the aqueous extract of M. oleifera. The cell viability test by trypan blue showed viable cells only at the tested concentrations of 100 µg mL (85.6%) and 50 µg mL (95.5%). In this context, the antimutagenic effect presented by the aqueous extract of M. oleifera may be the result of a synergistic action between the various constituents of the plant and its cytotoxic action depends on the concentration used. Under the conditions carried out, the study suggests that the aqueous extract of M. oleifera  has no cytotoxic action at low concentrations and has a chemoprotective effect for cancer.

References

  1. ABDULL, R.A.F., IBRAHIM, M.D., KNTAYYA, S.B. Health benefits of Moringa oleifera. Asian. Pac. J. Cancer Prev. v.15(20), p. 8571-6, 2014. doi: 10.7314/apjcp.2014.15.20.8571
  2. ALMEIDA, I. L. S. Avaliação da capacidade de adsorção da torta de Moringa oleifera para BTEX em amostras aquosas. 2010. 88 f. Dissertação (Mestrado em Ciências Exatas e da Terra) - Universidade Federal de Uberlândia, Uberlândia, 2010.
  3. AMAGLO, N.K., BENNETT, R.N., LO CURTO, R.B., ROSA, E.A.S., LO TURCO, V., GIUFFRIDA, A., TIMPO, G.M. Profiling selected phytochemicals and nutrients in different tissues of the multipurpose tree Moringa oleifera L., grown in Ghana. Food Chem. v.122, p.1047-1054, 2010. https://doi.org/10.1016/j.foodchem.2010.03.073
  4. ANVISA. RESOLUÇÃO-RE Nº 1.478, DE 3 DE JUNHO DE 2019. Proibidos alimentos com Moringa oleifera. ANVISA. Disponível em: http://portal.anvisa.gov.br/noticias/-/asset_publisher/FXrpx9qY7FbU/content/proibidos-alimentos-com-moringa-oleifera/219201. Acesso em: junho de 2019.
  5. ANWAR, F.; LATIF, S.; ASHRAF, M.; GILANI, A.H. Moringa oleifera: A food plant with multiple medicinal uses. Phytother. Res. v. 21, p. 17-25, 2007.
  6. doi: 10.1002/ptr.2023.
  7. AWODELE, O., OREAGBA, I.A., ODOMA, S., SILVA, J.A.T., OSUNKALU, V.O. Toxicological evaluation of the aqueous leaf extract of Moringa oleifera Lam. (Moringaceae). Journal of Ethnopharmacology, v. 139, p. 330–336, 2012. doi: 10.1016/j.jep.2011.10.008
  8. BALDISSEROTTO, A., BUSO, P., RADICE, H., DISSETTE, V., LAMPRONTI, E.U., GAMBARI, R., MANFREDINI, S., VERTUANI, S. Moringa oleifera leaf extracts as multifunctional ingredients for “Natural and Organic” Sunscreens and Photoprotective Preparations. Molecules, v. 23, p.664, 2018.
  9. doi: 10.3390/molecules23030664.
  10. BELLOSTAS, N., SORENSEN, J., NIKIEMA, A., SORENSEN, H., PASTERNAK, D., KUMAR, S. Glucosinolates in leaves of Moringa species grown and disseminated in Niger. African J. Agr. Res., v. 5, p.1338-1340, 2010.
  11. BEZERRA, A., MOMENTÉ, V.G., MEDEIROS, F. S. Germinação de sementes e desenvolvimento de plântulas de moringa (Moringa oleifera Lam.) em função do peso da semente e do tipo de substrato. Hort. Bras., v.22, p. 295-299, 2004. https://doi.org/10.1590/S0102-05362004000200026.
  12. COLOMBO, M.L., ASSISI, F., DELLA, P. T., MORO, P., SESANA, F.M., BISSOLI, M., BORGHINI, R., PEREGO, S., GALASSO, G., BANFI, E., DAVANZO, F. Most commonly plant exposures and intoxications from outdoor toxic plants. J. Pharm. Sci. Res., v.2, n.7, p.417-25, 2010
  13. ELISABETSKY, E. Etnofarmacologia como ferramenta na busca de substâncias ativas. In: SIMÕES, C. M. O.; SCHENCKEL, E. P.; GOSMANN, G.; MELLO, J.C.P.; MENTZ, L. A. & PETROVICK, P.R.: Farmacognosia: da planta ao medicamento. 3ª ed. Porto Alegre: Editora UFRGS/UFSC RS. 833 p., 2001.
  14. FENECH, M. The in vitro micronucleus technique. Mutat. Res. v. 455, p. 81–95, 2000.
  15. doi: 10.1016/s0027-5107(00)00065-8
  16. GOYAL, B.R., AGRAWAL, B.B., GOYAL, R.K., MEHTA, A. A. Phyto-pharmacology de Moringa oleifera Lam. an overview. Nat. Prod. Rad., v. 6, p. 347-353, 2007.
  17. GUEVARA, A.P., VARGAS, C., SAKURAI, H., FUJIWARA, Y., HASHIMOTO, K., MAOKA, T., KOZUKA, M.; ITO, Y., TOKUDA, H., NISHINO, H. An antitumor promoter from Moringa oleifera Lam. Mutat. Res. v.440, p. 181-188. 1999.
  18. doi: 10.1016/s1383-5718(99)00025-x
  19. JUNG, I. L., LEE, J. H., KANG, S. C. A potential oral anticancer drug candidate, Moringa oleifera leaf extract, induces the apoptosis of human hepatocellular carcinoma cells. Oncol. Lett. Sep. v. 3, p. 1597–1604, 2015. doi: 10.3892/ol.2015.3482
  20. KHAWAJA, T.M., TAHIRA, M., IKRAM, U.K. Moringa oleifera: a natural gift - A review. J. Pharm. Sci. Res. v. 2, p. 775-81, 2010.
  21. MacGREGOR, J. T., HEDDLE, J.A., HITE, M., MARGOLIN, B. H., RAMEL, C., SALAMONE, M.F., TICE, R.R., WILD, D. Guidelines for the conduct of micronucleus assay in mammalian bone marrow erythrocytes. Mut. Res. v.189, p.103-112, 1987. https://doi.org/10.1016/0165-1218(87)90016-4
  22. MAKKAR, H.P.S., BECKER, K. Nutritional value and antinutritional components of whole and ethanol extracted Moringa oleifera leaves. Anim. Feed Sci. Tech. v. 63, p. 211-228, 1996. https://doi.org/10.1016/S0377-8401(96)01023-1
  23. MANOHARAN, K., BANERJEE, M. β-Carotene reduces sister chromatid exchange induce chemical carcinogens in mouse mammary cells in organ culture. Cel.l Biol. Int. Rep. v. 9, n. 8, p. 783-789, 1985.
  24. doi: 10.1016/0309-1651(85)90096-7.
  25. MATOS, F.J.A.: Farmácias vivas: sistema de utilização de plantas medicinais projetado para pequenas comunidades. 4ª ed. Fortaleza: UFC, SEBRAE/CE, 2002.
  26. NAIR, S., VARALAKSHMI, K. Anticancer, cytotoxic potential of Moringa oleifera extracts in HeLa cell line. J. Nat. Pharm. v. 2, p. 138-138. 2011.
  27. doi:10.4103/2229-5119.86260
  28. NEVES, S. M. P., ONG, F. M. P., RODRIGUES, L. D., SANTOS, R. A., FONTES, R. S., SANTANA, R. O. Manual de Cuidados e Procedimentos com Animais
  29. de Laboratório do Biotério de Produção e Experimentação da FCF-IQ/USP. 2013. 216 p. il. São Paulo: FCF-IQ/USP, 2013.
  30. PEREIRA, C.A.B. Teste estatístico para comparar proporções em problemas de citogenética. In: RABELLO-GAY, M.N., RODRIGUES, M.A, La, R., Montelleone-Neto (Eds.) Mutagênese, teratogênese e carcinogênese: métodos e critérios de avaliação. São Paulo: FCA, p.113-21, 1991.
  31. PROMKUM, C., KUPRADINUN, P., TUNTIPOPIPAT, S., BUTRYEE, C. Nutritive evaluation and effect of Moringa oleifera pod on clastogenic potential in the mouse. Asian Pac. J. Cancer Prev. v.11, p.627-632, 2010.
  32. PURWAL, L., PATHAK, A.K., JAIN, U.K. In vivo anticancer activity of the leaves and fruits of Moringa oleifera on mouse melanoma. Pharmacology online. v. 1, p. 655–665, 2010.
  33. SALVADORI, D.M.F., RIBEIRO, L.R., NATARAJAN, A.T. Effect of beta-carotene on clastogenic effects of mitomycin C, methyl methansulfonate and bleomycin in CHO cells. Mutat. Res., v.9, p.53-57, 1994.
  34. doi: 10.1093/mutage/9.1.53.
  35. SATHYA, T.N., AADARSH, P., DEEPA, V., BALAKRISHNA, M. P. Moringa oleifera Lam. leaves prevent cyclophosphamide-induced micronucleus and DNA damage in mice. Int. J. Phytomed. v. 2, p.147–154, 2010. doi:10.5138/ijpm.2010.0975.0185.02023
  36. SIDKER, K., SINHA, M., DAS, N., DAS, D.K., DATTA, S., DEY, S. Moringa oleifera Leaf extract prevents in vitro oxidative DNA damage. Asian J. Pharm. Clin. Res. v. 6, p.159–163, 2013.
  37. SILVA, J., ERDTMANN, B., HENRIQUES, J.A.P. Genética Toxicológica. Porto Alegre: Alcance. 344 p., 2003.
  38. SILVA, G. N., DE CAMARGO, E. A., SALVADORI, D. M.; RIBEIRO, D. A. Genetic damage in human peripheral lymphocytes exposed to antimicrobial endodontic gents. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endontology, v.104, p. 58-61, 2007. doi: 10.1016/j.tripleo.2007.02.009
  39. SREELATHA, S., JEYACHITRA, A., PADMA, P.R. Antiproliferation and induction of apoptosis by Moringa oleifera leaf extract on human cancer cells. Food Chem. Toxicol. v. 49, p. 1270–1275, 2011. doi: 10.1016/j.fct.2011.03.006
  40. SUGUI, M.M. Mecanismos de antimutagenicidade do cogumelo Agaricus brasiliensis sobre lesões no DNA induzidas in vivo e in vitro. 111 f. Tese de Doutorado (Doutorado em Patologia) - Faculdade de Medicina – UNESP – Botucatu- São Paulo, Brasil, 2006.
  41. TÔRRES, A.R., OLIVEIRA, R.A.G., DINIZ, M.F.F.M., ARAUJO, E.C. Estudo sobre o uso de plantas medicinais em crianças hospitalizadas da cidade de João Pessoa: riscos e benefícios. Rev. Bras. Farmacogn., v.15, n.4, p.373-380, 2005. https://doi.org/10.1590/S0102-695X2005000400019.
  42. WATERS, M.D., BRADY, A. L., STACK, H. F., BROCKMAN, H. E. Antimutagenic profiles for some model compounds. Mut. Res. v. 238, p. 57-85, 1999. doi: 10.1016/0165-1110(90)90039-e.

How to Cite

Ferreira, W. A. da S. ., Pereira, E. G. da S. ., Aguiar, D. H. ., Oliveira, A. F. de ., & Sugui, M. M. . (2022). Evaluation of the biological effect of Moringa oleifera Lam. in vivo. Scientific Electronic Archives, 16(1). https://doi.org/10.36560/16120231731