Resumo
Circa 80% of the neotropical species of Asclepiadoideae (Apocynaceae) belong to the “MOOG Cladeâ€, whose interrelations are poorly established. This study describes the floral morphology,
anther development, microsporogenesis, microgametogenesis, and pollinarium and pollen grain morphology of
Oxypetalum appendiculatum to foster future taxonomic work. In addition to typical morphological features of Asclepiadoideae, as a bifid vinaceous appendix and a translator with a laterally expanded retinaculum, flowers present a clear division of stamens into three groups, with respect to size and arrangement in the flower, which has not yet been reported in the literature. Stamens present connate, subsessile filaments, bisporangiate anthers with six parietal layers, and dicotyledonous development. Endothecium is unthickened; secretory tapetum is biseriate on the adaxial side and multiseriate on the abaxial surface. Successive microsporogenesis produces linear tetrads. Single pollen grains, united in pollinia, are tricellular and inaperturate. Features as bisporangiate anthers and successive microsporogenesis delimit the subfamily. Unthickened endothecium may indicate a derived position of
O. appendiculatum in the “MOOG Cladeâ€.
Referências
- CIVEYREL, L.; ROWE, N. Phylogenetic relationships of Secamonoideae based on the plastid gene matK, morphology, and biomechanics. Annals of the Missouri Botanical Garden. v. 88, p. 583-602, 2001.
- COCUCCI, A.A.; MARINO, S.; BARANZELLI, M.; WIEMER, A.P.; SERSIC, A. The buck in the milkweed: evidence of male-male interference among pollinaria on pollinators. New Phytologist. v. 203, p. 280-286, 2014.
- CORRY, T.H. On the mode of development of the pollinium in Asclepias cornuti, Decaisne. Transactions of the Linnean Society of London. v. 2, n. 75-84, 1882.
- DEMARCO, D. Micromorphology and histochemistry of the laticifers from vegetative organs of asclepiadoideae species (Apocynaceae). Acta Biológica Colombiana. v. 20, p. 57-65, 2015.
- DICKO-ZAFIMAHOVA, L. Etude ontogenique de la pollinie de Calotropis procera (Asclepiadaceae): apport de la microscopie photonique. Grana. v. 19, p. 85-98, 1980.
- ENDRESS, P.K. Development and evolution of extreme synorganization in angiosperm flowers and diversity: a comparison of Apocynaceae and Orchidaceae. Annals of Botany. v. 117, p. 749–767, 2016.
- ENDRESS, M.E.; BRUYNS, P.V. A revised classification of the Apocynaceae s.l. The Botanical Review. v. 66, p. 1-56, 2000.
- ENDRESS, M.E.; LIEDE-SCHUMANN, S.; MEVE, U. An updated classification for Apocynaceae. Phytotaxa. v. 159, p. 175-194, 2014.
- FOURNIER, E. Asclepiadaceae in Martius. Flora Brasiliensis. v. 6, p. 189-332, 1885.
- FYRE, T.C.; BLODGETT, E.B. A contribution to the life history of Apocynum androsamifolium. Botanical Gazette. v. 40, p. 49-53, 1905.
- GHIMIRE, B.; GHIMIRE, B.K.; HEO, K. Microsporogenesis in Rauvolfia serpentina (L.) Benth ex Kurz (Apocynaceae): an evidence for dual cytokinesis in microspore mother cells. Journal of Medicinal Plants Research. v. 5, p. 432-438, 2011.
- GUIGNARD, L. The egg in Apocynaceae and Asclepiadaceae. Review of Scientific Instruments. v. 55, p. 1-3, 1917.
- HARDER, L.D.; AIZEN, M.A. Floral adaptation and diversiï¬cation under pollen limitation. Proceedings of the Royal Society B: Biological Sciences. v. 365, p. 529-543, 2010.
- HARDER, L.D.; JOHNSON, S.D. Function and evolution of aggregated pollen in angiosperms. The International Journal of Plant Sciences. v. 169, p. 59-78, 2008.
- HARDER, L.D.; ROUTLEY, M.B. Pollen and ovule fates and reproductive performance by flowering plants. In: LD Harder, SCH Barrett, eds. Ecology and evolution offlowers. Oxford: Oxford Universit Press. 2006.
- JUDD, W.S.; CAMPBELL, C.S.; KELLOG, E.A.; STEVENS, P.F.; DONOGHUE, M.J. Sistemática vegetal: um enfoque filogenético. Artmed. 2009.
- KARNOVSKY, M.J.A. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. The Journal of Cell Biology. v. 27, p. 137-138, 1965.
- KUNZE, H. Morphology of the stamen in the Asclepiadaceae and its systematic relevance. Botanische Jahrbücher für Systematik. v. 118, p. 547-579, 1996.
- KUNZE, H. Ontogeny of the translator in the Asclepiadaceae s. str. Plant Systematics and Evolution. v. 193, p. 223-242, 1994.
- KOTOVSKI, E.R. Morfoanatomia floral em Allamanda L. (Apocynaceae, Rauvolfioideae). PhD Thesis, State University of Campinas, São Paulo. 2013.
- LAMBA, L.C. Microsporogenesis and male gametophyte in Rauvolfia serpentina (L.) Benth. ex. Kurz. Agriculture, Biology & Environmental Sciences. v. 45, p. 387-388, 1976.
- LIEDE, S. Anther differentiation in the Asclepiadaceae-Asclepiadeae form and function. In: D’ARCY, W. G.; KEANTING, R. C. (Ed.). The anther: form, function and phylogeny. Cambridge: Cambridge University Press. 1996.
- LIEDE-SCHUMANN, S.; NIKOLAUS, M.; SILVA, U.C.S.; RAPINI, A.; MANGELSDORFF, R.D.; MEVE, U. Phylogenetics and biogeography of the genus Metastelma (Apocynaceae-Asclepiadoideae-Asclepiadeae: Metastelmatinae). Systematic Botany. v. 39, p. 594-612, 2014.
- LIEDE-SCHUMANN, S.; RAPINI, A; GOYDER, D.J.; CHASE, M.W. Phylogenetics of the new world subtribes of Asclepiadeae (Apocynaceae-Asclepiadoideae): Metastelmatinae, Oxypetalinae, and Gonolobinae. Systematic Botany. v. 30, p. 183-194, 2005.
- MAHESWARI, D.H. Embryology of Rauvolfia serpentina and Catharanthus roseus. Agriculture, Biology & Environmental Sciences. v. 39, p. 376-377, 1970.
- MANNING, J.C. Diversity of endothecial patters in the angiosperms. In: D’ARCY, W.G.; KEATING, R. C. The anther: form, function and phylogeny. Cambridge: Cambridge University Press. 1996.
- MEYER, S. Studies in the family Apocynaceae. Journal of Separation Science. v. 1, p. 131-158, 1938.
- O’BRIEN, T.P.; FEDER, N.; MCCULLY, M.E. Polychromatic staining of plant cell walls by toluidine blue O. Protoplasma. v. 59, p. 368-373, 1964.
- PACINI, E. Relationship between tepetum, loculus, and pollen during development. International Journal of Biological Sciences. v. 171, p. 1-11, 2010.
- PACINI, E.; FRANCHI, G.G.; HESSE, M. The tapetum: its form, function, and possible phylogeny in Embryophyta. Plant Systematics and Evolution. v. 149, p. 155-185, 1985.
- PAIVA, J.G.A.; FANK-DE-CARVALHO, S.M.; MAGALHÃES, M.P.; GRACIANO-RIBEIRO, D. Verniz vitral incolor 500®: uma alternativa de meio de montagem economicamente viável. Acta Botanica Brasilica. v. 20: p. 257-264, 2006.
- POTGIETER, K.; ALBERT, V.A. Phylogenetic relationships within Apocynaceae s.l. based on trnL intron and trnL-F spacer sequences and propagule characters. Annals of the Missouri Botanical Garden. v. 88, p. 523-549, 2001.
- RAPINI, A.; CHASE, M.W.; GOYDER, D.J.; GRIFFITHS, J. Asclepiadeae classification: evaluating the phylogenetic relationships of New World Asclepiadoideae (Apocynaceae). Taxon. v. 52, p. 33-50, 2003.
- RAPINI, A. Taxonomy “under constructionâ€: advances in the systematics of Apocynaceae, with emphasis on the Brazilian Asclepiadoideae. Rodriguésia. v. 63, p. 75-88, 2012.
- RAU, M.A. Studies in Apocynaceae. The Indian Botanical Society. v. 19, p. 33-44, 1940.
- SCHILL, R.; JAKEL, U. Beitrag zur Kenntnis der Asclepiadaceen-Pollinarien. Tropische und Subtropische Pflanzenwelt. v. 22, p. 53-170, 1978.
- SCHUMANN, K. Apocynaceae & Asclepiadaceae. In: Die natürlichen Pflanzenfamilien (HGA Engler, KAE Prantl, eds.). Leipzig: Wilhelm Engelmann. 1895.
- SILVA, N.M.F; VALENTE, M.DAC.; PEREIRA, J.F.; FILHO, G.M.A.; ANDRADE, L.R. Morfoanatomia de espécies brasileiras de Oxypetalum (Asclepiadoideae-Apocynaceae). Rodriguésia. v. 59, p. 915-948, 2008.
- SIMÕES, A.O.; CASTRO, M.M.; KINOSHITA, L.S. Calycine colleters of seven species of Apocynaceae (Apocynoideae) from Brazil. Biological Journal of the Linnean Society. v. 152, p. 387-398, 2006.
- SINHA, S.; MONDAL, A.K. The morphological diversification of pollinia of some members of Asclepiadaceae. African Journal of Biotechnology. v. 10, p. 7981-7986, 2011.
- SUD, K.C. Contribution to the embryology of Trachelospermum fragrans Hook. F. (Apocynaceae). Plant Science. v. 93, p. 495-501, 1984.
- SWARUPANANDAN, K.; MANGALY, J.K.; SONNY, T.K.; KISHOREKUMAR, K.; BASH, C.S. The subfamilial and tribal classification of the family Asclepiadaceae. Biological Journal of the Linnean Society. v. 1220, p. 327-369, 1996.
- VALENTE, M.C. A flor de Oxypetalum banskii Roem. et Schult. subsp. banskii: Estudo da anatomia e vascularização (Asclepiadoideae). Rodriguésia. v. 29, p. 161-283, 1977.
- VALENTE, M.C.; COSTA, C.G. Estudo anatômico da flor de Marsdenia loniceroides E. Fournier (Asclepiadoideae-Apocynaceae). Rodriguésia. v. 56, p. 51-66, 2005.
- VERHOEVEN, R.L.; VENTER, H.J.T. Pollen morphology of the Periplocoideae, Secamonoideae and Asclepiadoideae (Apocynaceae). Annals of the Missouri Botanical Garden. v. 88, p. 569-582, 2001.
- VERHOEVEN, R.L.; VENTER, H.J.T. Pollinium structure in Periplocoideae (Apocynaceae). Grana. v. 37, p. 1-14, 1998.
- VIEIRA, M.F.; SHEPHERD, G.J. Oxypetalum banksii subsp. banksii: a taxon of Asclepiadaceae with an extra gynoecial compitum. Plant Systematics and Evolution. v. 233, p. 199-206, 2002.