Ir para o conteúdo principal Ir para o menu de navegação principal Ir para o rodapé
Scientific Electronic Archives
Ciências Biológicas
DOI: 10.36560/13920201190
Enviado: 2020-06-16
Publicado: 2020-09-01

Evaluation of antibacterial activity from different Aloe vera (L.) Burm. F. extracts against Staphylococcus aureus strains

Universidade Federal de Mato Grosso
bovine mastitis bacteria agar disk-diffusion agar well diffusion Aloe vera.

Resumo

This study aimed to evaluate the susceptibility of Staphylococcus aureus strains isolated from the milk of cows with subclinical mastitis to Aloe vera in natura sap, ethanolic extract and in natura gel. The substances were tested using agar well diffusion and agar disk-diffusion methods. There was no inhibition of bacterial growth in the agar disk-diffusion method. On the other hand, the agar well diffusion method displayed in vitro growth inhibition of S. aureus in ethanolic extract and in natura sap of 91.66% (22/24) and 35.29% (6/17), respectively.  Although the gel showed no effect against the strains tested in both methods, the agar well diffusion method was the most efficient to evaluate the antimicrobial activity of Aloe vera extracts.

Referências

  1. AGARRY, OO., OLALEYE, MT., BELLO-MICHAEL, CO. Comparative antimicrobial activities of Aloe vera gel and leaf. African journal of biotechnology. 2005;4(12):1413-1414.
  2. AMARO, LPA., MACIEL, MV., LUCENA, JA., LIMA JÚNIOR, DM., SOMBRA, DS. Utilização do extrato aquoso da babosa (Aloe vera) no manejo higiênico de ordenha em cabras. Agropecuária Científica no Semiárido. 2011;7(1):06-10.
  3. BANU, A., SATHYANARAYANA, BC., CHATTANNAVAR, G. Efficacy of fresh Aloe vera gel against multi-drug resistant bacteria in infected leg ulcers. The Australasian Medical Journal. 2012;5(6):305-309.
  4. BRASIL. Agência Nacional de Vigilância Sanitária. Farmacopeia Brasileira, volume 2. Brasília: Anvisa, 2010. 904p.
  5. BUSSMANN, RW., MALCA-GARCÃA, G., GLENN, A., SHARON, D., CHAIT, G., DÃAZ, D., POURMAND, K., JONAT, B., SOMOGY, S., GUARDADO, G., AGUIRRE, C., CHAN, R., MEYER, K., KUHLMAN, A., TOWNESMITH, A., EFFIO-CARBAJAL, J., FRÃAS-FERNANDEZ, F., BENITO, M. Minimum inhibitory concentrations of medicinal plants used in Northern Peru as antibacterial remedies. Journal of Ethnopharmacology. 2010;132(1):101-108.
  6. CARDOSO, FL., MURAKAMI, C., MAYWORM, MAS., MARQUES, LM. Análise sazonal do potencial antimicrobiano e teores de flavonoides e quinonas de extratos foliares de Aloe arborescens Mill. Xanthorrhoeaceae. Revista Brasileira de Farmacognosia. 2010;20(1):35-40.
  7. CASTRO, LO., RAMOS, RLD. Cultivo de três espécies de babosa: descrição botânica e cultivo de Aloe arborescens Mill. babosa-verde, Aloe saponaria (Aiton) Haw. babosa-listrada e Aloe vera L. Burm. f., babosa-verdadeira ou aloe-de-curaçau (ALOEACEAE). Porto Alegre: FEPAGRO; 2002. 11p. (Fundação Estadual de Pesquisa Agropecuária, Circular Técnica, 20).
  8. CHIKWANDA, D., NYAMUSHAMBA, GB., MATONDI, GHM., MARANDURE, T., CHIKWANDA, AT., IMBAYARWO-CHIKOSI, VE., MASUNDA, B., MASAMA, E., CHIWARA, A. Bacteriocidal efficacy of Aloe vera (Burm L.) against mastitis pathogens Streptococcus agalactiae, Staphylococcus aureus and Escherichia coliin vitro. Science. 2013;2(10):1-3.
  9. CLSI. Performance Standarts for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement. CLSI document M100-S22. Wayne, PA: Clinical and Laboratory Standards Institute. 2012. 188p.
  10. GLOBBO-NETO, L., LOPES, NP. Plantas medicinais: fatores de influência no conteúdo de metabólitos secundários. Química Nova. 2007;30(2):374-381.
  11. GRANDI, TSM. Tratado das plantas medicinais mineiras, nativas e cultivadas. 1st ed. digital. Belo Horizonte: Adaequatio Estúdio; 2014.p.1204.
  12. HABEEB, F., SHAKIR, E., BRADBURY, F., CAMERON, P., TARAVATI MR, DRUMMOND, AJ., GRAY, AI., FERRO, VA. Screening methods used to determine the anti-microbial properties of Aloe vera inner gel. Methods. 2007;42(4):315-320.
  13. HAMMAN, JH. Composition and applications of Aloe vera leaf gel. Molecules. 2008;13(8):1599-1616.
  14. KEDARNATH, KAMBLE KAVERI, M., VISHWANATH, BC., PATIL, CS. Antimicrobial activity of Aloe vera leaf extract. International Journal of Applied Biology and Pharmaceutical Technology. 2013;4(4):286-290.
  15. KONEMAN, EW., ALLEN, SD., JANDA, WM. Diagnósticos microbiológicos: texto e atlas colorido. 5th. Rio de Janeiro, RJ: MEDSI: 2008. p. 1760.
  16. LORENZI, H., SOUZA, HM. Plantas ornamentais no Brasil: arbustivas, herbáceas e trepadeiras. 4th ed. Nova Odessa: Plantarum; 2008. p. 326.
  17. MADDISON, JE., WATSON, DJ., ELLIOT, J. Medicamentos antibacterianos. In Maddison JE, Page SW, Church DB. Farmacologia Clínica de Pequenos Animais. 2nd ed. Rio de Janeiro: Elsevier; 2010. p.147-184.
  18. MOODY, JO., ADEBIYI, OA., ADENIYI, BA. Do Aloe vera and Ageratum conyzoides enhance the anti-microbial activity of traditional medicinal soft soaps (Osedudu)? Journal of ethnopharmacology. 2004;92(1):57-60.
  19. RAMOS, AP., PIMENTEL, LC. Ação da Babosa no reparo tecidual e cicatrização/Effectiveness of Aloe vera on the tissue repair and healing process. Brazilian Journal of Health. 2011;2(1):40-48.
  20. REYNOLDS, T., DWECK, AC. Aloe vera leaf gel: a review update. Journal of ethnopharmacology. 1999;68(1):3-37.
  21. SARTÓRIO, ML. Cultivo orgânico de plantas medicinais. 1st ed. Viçosa: Aprenda Fácil Editora; 2000. p.258.
  22. SEMENOFF SEGUNDO, A., BOSCO, AF., MAIA, D., RIBEIRO, RV., AGUIAR, EBH., ROCATTO, GEGD., CIRILO, DM., BRUZELLE, SL., SEMENOFF, TADV. Influência do Aloe vera e Própolis na contração de feridas em dorso de ratos. Revista Periodontia. 2007;17(1):05-10.
  23. SEMENOFF, TADV., FERREIRA, WRS., SEMENOFF-SEGUNDO, A., BIASOLI, ER. Efetividade in vitro de Aloe vera in natura, gel de clorexidina a 0,12% e gel de clorexidina a 2% sobre Enterococcus faecalis. Revista Odonto Ciência. 2008;23(3):283-286.
  24. SHILPAKALA, SR., PRATHIBA, J., MALATHI, R. Susceptibilities of Escherichia coli and Staphylococcus aureus to Aloe barbadensis. European review for medical and pharmacological sciences. 2009;13(6):461-464.
  25. SILVEIRA, LMS., OLEA, RSG., MESQUITA, JS., CRUZ, ALN., MENDES, JC. Metodologias de atividade antimicrobiana aplicadas a extratos de plantas: comparação entre duas técnicas de ágar difusão. Revista Brasileira de Farmacognosia. 2009 90(2):124-128.
  26. SOCIEDADE BRASILEIRA DE FARMACOGNOSIA. Antraquinonas[Internet]. Sociedade Brasileira de Farmacognosia; [cited 2015 Apr 20]. Available from: http://www.sbfgnosia.org.br/Ensino/antraquinonas.html.
  27. STANLEY, MC., IFEANYI, OE., EZIOKWU, OG. Antimicrobial effects of Aloe vera on some human pathogens. International Journal of Current and applied sciences. 2014;3(3):1022-1028.
  29. THE INTERNATIONAL ALOE SCIENCE COUNCIL. Aloe vera Frequently Asked Questions [Internet]. The International Aloe Science Council. [cited 2018 Feb 19] Available from: http://www.iasc.org/Consumers/AloeVeraFAQ.aspx.
  30. THE LINNEAN SOCIETY OF LONDON. Botanical Journal of the Linnean Society, 2016;181:1–20.
  31. VALGAS, C., SOUZA, SM., SMÂNIA, EFA., SMÂNIA-JR, A. et al. Screening methods to determine antibacterial activity of natural products. Brazilian Journal of Microbiology. 2007;38(2):369-380.

Como Citar

Tesk, A., Freitas, F., Kemper, R. T., & Vieira, T. B. (2020). Evaluation of antibacterial activity from different Aloe vera (L.) Burm. F. extracts against Staphylococcus aureus strains. Scientific Electronic Archives, 13(9), 74–79. https://doi.org/10.36560/13920201190