Ir para o conteúdo principal Ir para o menu de navegação principal Ir para o rodapé
Ciências Agrárias
Publicado: 2019-04-01

In vitro activity of the Schinus terebinthifolius (R.) extract on engorged female tick of Rhipicephalus Boophilus microplus and on larvae hatchability

Ministério da Agricultura Pecuária e Abastecimento Laboratório Nacional Agropecuário/Lanagro-MG Laboratório de Controle de Produtos Biológicos

A. S. Dias

Doutorando do Programa de Pós Graduação do Departamento de Medicina Veterinária da UFV
Instituto Nacional de Tecnologia, Inovação e Meio Ambiente Conceição do Castelo, Espírito Santo State, Brasil

R. M. Spadetto

Instituto Nacional de Tecnologia, Inovação e Meio Ambiente

Conceição do Castelo,

Espírito Santo State,


Escola Família Agrícola de Belo Monte/Mimoso do Sul, ES Espírito Santo State, Brasil

C. A. Pinto

Escola Família Agrícola de Belo Monte/Mimoso do Sul, ES

Espírito Santo State,


SEBRAE-COOPTTEC Espírito Santo State, Brasil

C. J. M. Barros

Espírito Santo State,

Tick Control Phytotherapy Schinus terebinthifolius Rhipicephalus Boophilus microplus


Ticks are the main ectoparasites responsible for losses in cattle livestock due to spoliation, hematozoa transmission, and destruction of the leather; Rhipicephalus Boophilus microplus is the main agent in tropical countries. The control of these agents is done basically with chemical products, but this procedure has presented some challenges, such as the rapid development of ticks' resistance against them. In this context, the phototherapy is an important alternative for controlling these agents. Schinus terebinthifolius, known as Brazilian peppertree, presents antimicrobial, antifungal, and anti-inflammatory activities. Many organic molecules are reported as having effect on organisms extracted from this plant. This paper aimed to test the efficient activity of the S. terebinthifolius extract under different concentrations on the egg mass formation of R. B. microplus and on larvae hatchability in vitro assay. Engorged females of R. B. microplus were collected in a bovine herd and were taken to laboratory and submitted to immersion test into alcoholic extract at 100%, 50%, 25%, 10%, 5%, and 2.5% dilutions. Test in impregnated papers was carried out under the same concentrations to verify the extract efficacy that causes larval mortality. Control groups were composed, one with water, one with alcohol, and one with a commercial formulation containing cypermethrin, chlorpyrifos, and piperonyl butoxide. It was found that the extract at 70% hydroalcoholic solution showed acceptable efficacy in 50% and 100% dilutions. Result of the impregnated papers test showed 100% extract efficacy at 50% and 100% concentration. These results suggest this phytotherapic extract could be used for R. B. microplus control.


  1. Azevedo, C.F., Quirino, Z.G.M., Bruno, R.L.A. Estudo farmacobotânico de partes aéreas vegetativas de aroeira-vermelha (Schinus terebinthifolius Raddi, Anacardiaceae). Revista Brasileira de Plantas Médicas, 17:26-35, 2015.
  2. Bendaoud, H., Romdhane, M., Souchard, J.P., Cazaux, S., Bouajila, J. Chemical composition and anticancer and antioxidant activities of Schinus Molle L. and Schinus terebinthifolius Raddi berries essential oils. Journal of Food Science, 75: 466-72, 2010.
  3. Barbosa, C.S., Borges, L.M.F., Louly, C.C.B., Rocha, T.L., Sabóia-Morais, S.M.T., Miguita, C.H., Garcez W.S., Garcez, F.R. In vitro activity of 3β-O-tigloylmelianol from Guarea kunthiana A. Juss (Meliaceae) on oogenesis and ecdysis of the cattle tick Rhipicephalus (Boophilus) microplus (Canestrini) (Acari: Ixodidae). Experimental Parasitology 164: 5-11, 2016.
  4. Ceruks, M., Romoff, P., Fávero, O.A., Lago, J.H.G. Constituintes fenólicos polares de Schinus terebinthifolius Raddi (Anacardiaceae). Quimica Nova, 30:597-599, 2007.
  5. Chagas, A.C.S., Barros, L.D., Cotinguiba, F., Furlan, M., Giglioti, R., Oliveira, M.C.S., Bizzo, H.R. In vitro efficacy of plant extracts and synthesized substances on Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Parasitology Research, 110:295-303, 2011.
  6. Chagas, A.C.S., Oliveira, M.C.S., Giglioti, R., Santana, R.C.M., Bizzo, H.R., Gama, P.E., Chaves, F.C.M. Efficacy of 11 Brazilian essential oils on lethality of the cattle tick Rhipicephalus (Boophilus) microplus. Ticks and Tick-borne Diseases, 7:427-432, 2016.
  7. Csordas, B.G., Cunha, R.C., Garcia, M.V., da Silva, S.S., Leite, F.L., Andreotti, R. Molecular characterization of the recombinant protein RmLTIBmCG-LTB: Protective immunity against Rhipicephalus (Boophilus) microplus. Plos One, 13:e0191596, 2018.
  8. Domingues, L.F., Giglioti, R., Feitosa, K.A., Fantatto, R.R., Rabelo, M.D., Oliveira, M.C.S., Oliveira, G.P., Bechara, G.H., Chagas, A.C.S. In vitro activity of pineapple extracts (Ananas comosus, Bromeliaceae) on Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Experimental Parasitology, 134:400-404, 2013.
  9. Fouche, G., Sakong, B.M., Adenubi, O.T., Dzoyem, J.P., Naidoo, V., Leboho, T., Wellington, K.W., Eloff, J.N. Investigation of the acaricidal activity of the acetone and ethanol extracts of 12 South African plants against the adult ticks of Rhipicephalus turanicus. Microscopy Research and technique Onderstepoort Journal Veterinary Research, 84:e1-e6, 2017.
  10. Kumar, K.G.A., Sharma, A.K., Kumar, S., Ray, D.D., Rawat, K.S., Srivastava, S., Ghosh, S. Comparative in vitro anti-tick efficacy of commercially available products and newly developed phyto-formulations against field collected and resistant tick lines of Rhipicephalus (Boophilus) microplus. Journal of Parasitic Diseases 40:1590-1596, 2016.
  11. Lenz, M., Orth, A.I. Fenologia reprodutiva, morfologia e biologia floral de Schinus terebinthifolius Raddi (Anacardiacease), em restinga da ilha de Santa Catarina, Brasil. Biotemas 17:67-89, 2004.
  12. Madzimure, J., Nyahangare, E.T., Hamudikuwanda, H., Hove, T., Stevenson, P.C., Belmain, S.R., Mvumi, B.M. Acaricidal efficacy against cattle ticks and acute oral toxicity of Lippia javanica (Burm F.) Spreng. Tropical Animal Health and Production, 43:481–489, 2011.
  13. Peixoto, E.C.M., Figueiredo, A., Porto, E.P., Silva, L.P., Santos, V.H., Silva, R.M. Application of Cymbopogon winterianus Jowitt and Azadirachta indica A. Juss in the control of Rhipicephalus (Boophilus) microplus. Journal of Medicinal Plants Research, 7:2392-2398, 2013.
  14. Rajabpour, A,, Mashhadi, A.R.A., Ghorbani, M.R. Acaricidal and repellent properties of some plant extracts against poultry red mite, Dermanyssus gallinae (Mesostigmata: Dermanyssidae). Persian Journal Acarology, 7:85–91, 2018.
  15. Rosado-Aguilar, J.A., Rodríguez-Vivas, R.I., Borges-Argaez, R., Arjona-Cambranes, K.A. Acaricidal activity of Havardia albicans and Caesalpinia gaumeri methanolic leaf extracts on Rhipicephalus microplus and its toxicity to laboratory animals. Experimental and Applied Acarology 71:345–354, 2017.
  16. Sharifi-Rad, J., Sureda, A., Tenore, G.C., Daglia, M., Sharifi-Rad, M., Valussi, M., Tundis, R., Sharifi-Rad, M., Loizzo, M.R., Ademiluyi, A.O., Sharifi-Rad, R., Ayatollahi, S.A., Iriti, M.. Biological Activities of Essential Oils: From Plant Chemoecology to Traditional Healing Systems. Molecules 22:1-55, 2017.
  17. Shyma, K.P., Gupta, J.P., Ghosh, S., Patel, K.K., Singh, V. Acaricidal effect of herbal extracts against cattle tick Rhipicephalus (Boophilus) microplus using in vitro studies. Parasitology Research, 113:1919–1926, 2014.
  18. Singh, N.K., Jyoti , Vemu B, Singh H, Prerna M, Daundkar PS, Sharma SK.. In vitro acaricidal activity of Murraya koenigii (L.) Spreng (Rutaceae) extracts against synthetic pyrethroid-resistant Rhipicephalus (Boophilus) microplus. Parasitology Research, 114: 1531-1539, 2015.
  19. Souza, J.R.L., Remedio, R.N., Arnosti, A., Abreu, R.M.M., Camargo-Mathias, M.I. The effects of neem oil (Azadirachta indica A. JUSS) enriched with different concentrations of azadirachtin on the integument of semi-engorged Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) females. Microscopy Research and technique, 80:838–844, 2017.
  20. Vélez, J.P.L., Hernández, G.L., Guzmán, P.A., Guerra, C.A.E. Effect of plant extracts of Jatropha curcas and Anonna muricata on engorged females of common cattle tick Ripichephalus (Boophilus) microplus under laboratory conditions. Revista CES Medicina Veterinaria y Zootecnia, 12:21-32, 2017.
  21. Villarreal, J.P.V., Santos, P.R.D., Silva, M.A.M.P.D., Azambuja. R.H.M., Gonçalves, C.L., Escareño, J.J.H., Santos, T.R.B.D., Pereira, C.M.P., Freitas, R.A., Nascente, P.D.S. Evaluation of phytotherapy alternatives for controlling Rhipicephalus (Boophilus) microplus in vitro. Revista Brasileira de Parasitologia Veterinaria, 26:299-306, 2017.
  22. Vinturelle, R., Mattos, C. , Meloni, J., Nogueira, J., Nunes, M.J., Vaz Jr, I.S., Rocha, L., Lione, V., Castro, H.C., Chagas, E.F. In Vitro Evaluation of Essential Oils Derived from Piper nigrum (Piperaceae) and Citrus limonum (Rutaceae) against the Tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Biochemistry Research International, ID 5342947:1-9, 2017.

Como Citar

Dias, A. S., Spadetto, R. M., Pinto, C. A., & Barros, C. J. M. (2019). In vitro activity of the Schinus terebinthifolius (R.) extract on engorged female tick of Rhipicephalus Boophilus microplus and on larvae hatchability. Scientific Electronic Archives, 12(2), 64–69.