Toxicity and effects of combined agrochemical in Scaptotrigona bipunctata bees

Authors

  • T. O. Diniz Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, Paraná, Brasil
  • N. C. Pereira Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, Paraná, Brasil
  • B. G. Silva Departamento de Ciências Exatas, Escola Superior de Agricultura “Luiz de Queiroz” – ESALQ/USP, Piracicaba, São Paulo, Brasil
  • W. C. S. Pizzaia Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, Paraná, Brasil
  • F. G. M. Oliveira Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, Paraná, Brasil
  • A. A. S. Gigliolli Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, Paraná, Brasil
  • M. C. C. R. Takasusuki Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, Paraná, Brasil

DOI:

https://doi.org/10.36560/131220201258

Keywords:

Histology, SEM, CEC, stingless bees

Abstract

The commercial insecticide Fastac Duo is a combined insecticide, widely used in different crops, acting on insects, affecting both pests and pollinators, such as bees. In this study, the effects of sublethal concentrations of Fastac Duo in stingless bees Scaptotrigona bipunctata were evaluated. Worker forager bees were exposed to the insecticide and histochemical and morphological analyses were conducted after 24, 48 and 72 h of ingestion. Brain analysis of S. bipunctata revealed changes in the chromatin condensing state according to exposure time and insecticide concentration when compared to the control group. Morphological changes were observed in the midgut in all concentrations and exposure times, which may interfere in several physiological processes. In conclusion, although the concentrations used in the study did not cause high mortality, it induced changes in the internal morphology that can lead to changes in bee activity.

Author Biography

T. O. Diniz, Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, Paraná, Brasil

Universidade Estadual de Maringá – Departamento de Biotecnologia, Genética e Biologia Celular

References

ADAPAR. Agência de Defesa Agropecuária do Paraná. 2018. Disponível em: http://www.adapar.pr.gov.br/. Acesso em: 13 de março de 2020.

ARENA, M.; SGOLASTRA, F. A meta-analysis comparing the sensitivity of bees to pesticides. Ecotoxicology, 23: 324–334, 2014. doi: 10.1007 / s10646-014-1190-1

BARBOSA, D.B.; CRUPINSKI, E.F.; SILVEIRA, R.N.; LIMBERGER, D.C.H. As abelhas e seu serviço ecossistêmico de polinização. Revista Eletrônica Científica da UERGS, 3:694-703, 2017. doi: 10.21674/2448-0479.34.694-703

BRIDI, R.; LARENA, A.; PIZARRO, P. N.; GIORDANO, A.; MONTENEGRO, G. LC-MS/MS analysis of neonicotinoid insecticides: Residue findings in Chilean honeys. Ciência e Agroecologia, 42:51-57, 2018. doi: 10.1590/1413-70542018421021117

CAMARGO, J.M.F.; PEDRO, S.R.M. Meliponini Lepeletier, 1836. In: MOURE, J.S.; URBAN, D. eds. Melo GAR (Orgs) Catalogue of Bees (Hymenoptera, Apoidea) in the Neotropical Region. Curitiba, Brazil: UFPR; Online Version 2013. Disponível em: http://www.moure.cria.org.br/catalogue. Acesso em: 13, janeiro, 2020.

CATAE, A.F.; ROAT, T.C.; OLIVEIRA, R.A.; NOCELLI, R.C.F.; MALASPINA, O. Cytotoxic effects of thiamethoxam in the midgut and malpighian tubules of Africanized Apis mellifera (Hymenoptera: Apidae). Microscopy Research & Technique, 77: 274–281, 2014 doi: 10.1002/jemt.22339

DINIZ, T.O.; PEREIRA, N.C.; PIZZAIA, W.C.S.; SINÓPOLIS-GIGLIOLLI, A.A.; SILVA, B.G.; BORGES, Y.M.; GUEDES, T.A.; RUVOLO-TAKASUSUSKI, M.C.C. Toxicity and genetic analysis of bees Scaptotrigona bipunctata after contamination with insecticide acephate. Scientific Electronic Archives, 13: 8–17, 2020. doi: 10.36560/ 13820201157

FALCO, J.R.P.; HASHIMOTO, J.H.; FERMINO, F.; TOLEDO, V.A.A. Toxicity of thiamethoxam, behavioral effects and alterations in chromatin of Apis mellifera L, 1758 (Hymenoptera; Apidae). Research Journal of Agriculture and Biological Sciences, 6:823–828, 2010.

FARIA, A.B.C. Revisão sobre alguns grupos de inseticidas utilizados no manejo integrado de pragas florestais. Ambiência, 5: 345–357, 2006.

FREITAS, B.M.; PINHEIRO, J.N. Efeitos sub-letais dos pesticidas agrícolas e seus impactos no manejo de polinizadores dos agroecossistemas brasileiros. Oecologia Australis, 14:282-298, 2010. doi: 10.4257/oeco.2010.1401.17

LANDIM, C.C. Abelhas: morfologia e função de sistemas. São Paulo, Brazil: UNESP, 2009. 408p.

MORAES, S.S.; BATISTA, A.R.; VIANA, B.F. Avaliação da toxicidade aguda (DL50 e CL50) de inseticidas para Scaptotrigona tubiba (Smith) (Hymenoptera: Apidae): via de contato. Anais da Sociedade Entomológica do Brasil, 29:31–37, 2000. doi: 10.1590/S0301-80592000000100004

MOREIRA, D.R.; SINÓPOLIS-GIGLIOLLI, A.A.; FALCO, J.R.P.; FERREIRA-JÚLIO, A.H.; VOLNISTEM, E.A.; CHAGAS, F.; TOLEDO, V.A.A.; RUVOLO-TAKASUSUKI, M.C.C. Toxicity and effects of the neonicotinoid thiamethoxam on Scaptotrigona bipunctata lepeletier, 1836 (Hymenoptera: Apidae). Environmental Toxicology, 33:463–475, 2018. doi: 10.1002 / tox.22533

OLIVEIRA, R.A.; ROAT, T.C.; CARVALHO, S.M.; MALASPINA, O. Side-effects of thiamethoxam on the brain and midgut of the africanized honeybee Apis mellifera (Hymenopptera: Apidae). Environmental Toxicology, 29:1122–1133, 2014. doi: 10.1002 / tox.21842

PACÃFICO-DA-SILVA, I.; MELO, M.M.; SOTO-BLANCO, B. Efeitos tóxicos dos praguicidas para abelhas. Revista Brasileira de Higiene e Sanidade Animal, 10: 142–157, 2016 doi: 10.5935/1981-2965.20160013

PEREIRA, N.C. Toxicidade e análise genética de abelhas Scaptotrigona bipunctata Lepeletier, 1836 (Hymenoptera, Meliponini) contaminadas com inseticida cipermetrina. Maringá: Universidade Estadual de Maringá, 2017. 52 f. Dissertação (Mestrado em Genética e Melhoramento).

RAMÃREZ, V.M.; AYALA, R.; GONZÃLEZ, H.D. In: VIT, P.; PEDRO, S.; ROUBIK, D. (eds) Pot-Pollen in Stingless Bee Melittology. Springer, 139-153, 2018.

R DEVELOPMENT CORE TEAM. R: a language and environment for statistical computing. Vienna: R Foundation for Statistical Computing, Vienna, 2019.

ROAT, T.C.; CARVALHO, S.M.; NOVELLI, R.C.F.; SILVA-ZACARIN, E.C.M.; OALMA, M.S.; MALASPINA, O. Effects of sublethal dose of fipronil on neuron metabolic activity of africanized honeybees. Archives of Environmental Contamination and Toxicology, 64: 456–466, 2013. doi: 10.1007/s00244-012-9849-1

ROSSI, C.A.; ROAT, T.C.; TAVARES, D.A.; CINTRA-SOCOLOWSKI, P.; MALASPINA, O. Effects of sublethal doses of imidacloprid in malpighian tubules of africanized Apis mellifera (Hymenoptera, Apidae). Microscopy Research and Technique, 76:552–558, 2013. doi: 10.1002/jemt.22199

SANCHEZ-BAYO, F.; GOKA, K. Pesticide residues and bees – A risk assessment. PLoS ONE, 9:e94482, 2014. doi: 10.1371/journal.pone.0094482

SANTOS, M.A.T.; AREAS, M.A.; REYES, F.G. Piretroides – uma visão geral. Alimentos e Nutrição, 18:339-349, 2007.

SANTOS, S.A.; FERMINO, F.; MOREIRA, B.M.T.; ARAUJO, K.F.; FALCO, J.R.P.; RUVOLO-TAKASUSUKI, M.C.C. Critical electrolyte concentration of silk gland chromatin of the sugarcane borer Diatraea saccharalis, induced using agrochemicals. Genetics and Molecular Research, 13:7958–7964, 2014. doi: 10.4238 / 2014.Setembro 29.9

SOARES, H.M.; JACOB, C.R.O; CARVALHO, S.M.; NOCELLI, R.C.; MALASPINA, O. Toxicity of imidacloprid to the stingless bee Scaptotrigona postica Latreille,1807 (Hymenoptera: Apidae). Bulletin of Environmental Contamination and Toxicology, 94:675–680, 2015. doi: 10.1007 / s00128-015-1488-6

TAVARES, D.A.; ROAT, T.C.; CARVALHO, S.M.; SILVA-ZACARIN, E.C.M.; MALASPINA; O. In vitro effects of thiamethoxam on larvae of Africanized honey bee Apis mellifera (Hymenoptera: Apidae). Chemosphere,135:370–378, 2015. doi: 10.1016 / j.chemosphere.2015.04.090

THANY, S.H.; BOURDIN, C.M.; GRATON, J.; LAURENT, A.D.; MATHÉ-ALLAINMAT, M.; LEBRETON, J.; QUESTEL, J.Y.L. Similar Comparative Low and High Doses of Deltamethrin and Acetamiprid Differently Impair the Retrieval of the Proboscis Extension Reflex in the Forager Honey Bee (Apis mellifera). Insects, 6:805-814, 2015. doi: 10.3390 / insects6040805

TISON, L.; HAHN, M.L.; HOLTZ, S.; RÖßNER, A.; GREGGERS, U.; BISCHOFF, G.; MENZEL, R. Honey Bees’ Behavior Is Impaired by Chronic Exposure to the Neonicotinoid Thiacloprid in the Field. Environmental Science & Technology, 50:7218-7227, 2016. doi: 10.1021 / acs.est.6b02658

TOMÉ, H.V.V.; BARBOSA, W.F.; CORRÊA, A.S.; GONTIJO, L.M.; MARTINS, G.F.; GUEDES, R.N.C. Reduce-risk insecticides in Neotropical stingless bee species: impact on survival and activity. Annals of Applied Biology, 167:186–196, 2015. doi: 10.1111/aab.12217

VIDAL, B.C.; MELLO, M.L.S. Critical electrolyte concentration of DNA and nucleoprotein complexes in vitro. Acta Histochemica et Cytochemica, 22:471–478, 1989.

Downloads

Published

2020-11-30

How to Cite

Diniz, T. O., Pereira, N. C., Silva, B. G., Pizzaia, W. C. S., Oliveira, F. G. M., Gigliolli, A. A. S., & Takasusuki, M. C. C. R. (2020). Toxicity and effects of combined agrochemical in Scaptotrigona bipunctata bees. Scientific Electronic Archives, 13(12), 41–53. https://doi.org/10.36560/131220201258

Issue

Vol. 13 No. 12 (2020): Scientific Electronic Archives

Section

Agricultural Science

License

The journal reserves the right to make changes to the original rules, spelling and grammatical order, in order to keep the language of worship default, respecting, however, the style of the authors. The finals will be sent to authors. Articles published are the property of Scientific Electronic Archives magazine, becoming its total or partial reprint, subject to the express authorization of the direction of the journal. The original source of publication should be retained. The originals will not be returned to the authors. Opinions expressed by authors of articles are solely your responsibility.

Licença Creative Commons
This journal uses the License Creative Commons Atribuição 4.0 Internacional.

Crossref
Scopus
Google Scholar
Europe PMC

Most read articles by the same author(s)

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.